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Literature summary for 2.1.1.180 extracted from

  • Witek, M.A.; Conn, G.L.
    Functional dichotomy in the 16S rRNA (m1A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization (2016), Nucleic Acids Res., 44, 342-353.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.180

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Catenulispora acidiphila

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.03 M Bis-Tris (pH 5.5) and 25.5% (w/v) polyethylene glycol 3350 Catenulispora acidiphila

Protein Variants

Protein Variants Comment Organism
D21A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
D36A inactive Catenulispora acidiphila
D61A inactive Catenulispora acidiphila
E94A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
K115A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
R43A/R73A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
R66A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
S201A the mutant shows increased binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
S202A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
W113A the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
W113F the mutant shows increased binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
W203A the mutant shows increased binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila
W203F the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme Catenulispora acidiphila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28600
-
 x * 28600, full-length protein, SDS-PAGE Catenulispora acidiphila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine1408 in 16S rRNA Catenulispora acidiphila
-
 S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Catenulispora acidiphila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Catenulispora acidiphila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine1408 in 16S rRNA
-
 Catenulispora acidiphila S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 28600, full-length protein, SDS-PAGE Catenulispora acidiphila

Synonyms

Synonyms Comment Organism
16S rRNA (m1A1408) methyltransferase
-
 Catenulispora acidiphila
KAM
-
 Catenulispora acidiphila