Literature summary for 2.1.1.179 extracted from

Cubrilo, S.; Babic, F.; Douthwaite, S.; Maravic Vlahovicek, G.
The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site (2009), RNA, 15, 1492-1497.

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Cloned(Commentary)

Cloned (Comment)	Organism
introduction of a recombinant version of the Sgm methyltransferase gene from Micromonospora zionensis into an Escherichia coli strain that has a full complement of housekeeping methyltransferases. Analyses of the 16S rRNA shows that the m5C1407-specific housekeeping methyltransferase RsmF (YebU) is outcompeted by Sgm as the resistance methyltransferase gains access to its own m7G1405 target on the 30S ribosomal subunit. A single amino acid change in Sgm, which lowers the level of conferred resistance, reduces the ability of Sgm to interfere with RsmF methylation on the 30S subunit	Micromonospora zionensis

Cloned (Comment)

Organism

introduction of a recombinant version of the Sgm methyltransferase gene from Micromonospora zionensis into an Escherichia coli strain that has a full complement of housekeeping methyltransferases. Analyses of the 16S rRNA shows that the m5C1407-specific housekeeping methyltransferase RsmF (YebU) is outcompeted by Sgm as the resistance methyltransferase gains access to its own m7G1405 target on the 30S ribosomal subunit. A single amino acid change in Sgm, which lowers the level of conferred resistance, reduces the ability of Sgm to interfere with RsmF methylation on the 30S subunit

Micromonospora zionensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA	Micromonospora zionensis	-	S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Micromonospora zionensis	Q7M0R2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA	-	Micromonospora zionensis	S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
Sgm methyltransferase	-	Micromonospora zionensis

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Release 2023.1 (January 2023)