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Literature summary for 2.1.1.178 extracted from

  • Demirci, H.; Larsen, L.H.; Hansen, T.; Rasmussen, A.; Cadambi, A.; Gregory, S.T.; Kirpekar, F.; Jogl, G.
    Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus (2010), RNA, 16, 1584-1596.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.178

Cloned(Commentary)

Cloned (Comment) Organism
gene TTHA1387 Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
RsmF in a complex with cofactor S-adenosyl-methionine, three crystal structures, X-ray diffrcation structure determination and analysis at 1.3 A resolution Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
additional information construction of a null mutant strain in which TTHA1387 is inactivated by homologous recombination and insertion of a heat stable kanamycin-resistance gene Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine1400 in 16S rRNA Thermus thermophilus
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1400 in 16S rRNA Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1404 in 16S rRNA Thermus thermophilus
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA Thermus thermophilus
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SJI2 gene TTHA1387 or rsmF
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SJI2 gene TTHA1387 or rsmF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate recognition mechanism, overview Thermus thermophilus ?
-
 ?
additional information substrate recognition mechanism, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
S-adenosyl-L-methionine + cytosine1400 in 16S rRNA
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1400 in 16S rRNA RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1400 in 16S rRNA
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1400 in 16S rRNA RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1404 in 16S rRNA
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1404 in 16S rRNA RsmF methylates C1404 to about 35% with 16S rRNA and completely with 30S subunits Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
 ?

Subunits

Subunits Comment Organism
More comparison of Thermus thermophilus RsmB and RsmF protein sequences, secondary and tertiary structure, differences in substrate binding, overview Thermus thermophilus

Synonyms

Synonyms Comment Organism
16S rRNA methyltransferase
-
 Thermus thermophilus
RsmF
-
 Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 no difference in the relative growth proportions of the wild-type and rsmF mutant at 70°C. However, at 60°C, the rsmF null mutant constitutes only around 5% of the population, and at 80°C the rsmF null mutant is unable to grow at all Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Thermus thermophilus

General Information

General Information Comment Organism
metabolism two methyltransferases, RsmB and RsmF, are responsible for all four 5-methylcytidine modifications in 16S rRNA of Thermus thermophilus, overview. RsmB produces m5C967, while RsmF methylates C1400, C1404, and C1407 in a 30S subunit substrate, but only C1400 and C1404 when naked 16S rRNA is the substrate. C1400 and C1404 are sufficiently buried in the mature ribosome structure so require extensive unfolding of the rRNA to be accessible to RsmF Thermus thermophilus