Cloned (Comment) | Organism |
---|---|
gene rsmF, expression of wild-type enzyme in Escherichi coli Top10 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | RmtC, EC 2.1.1.179, in the presence of aminoglycosides impedes methylation at the N5 position of nucleotide C1407 when the N7 position of G1405 is methylated, MALDI mass spectrometry product analysis, mechanism, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | Escherichia coli | nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | Escherichia coli BW25113 | nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
i.e. strain BB1074, gene rsmF | - |
Escherichia coli BW25113 | - |
i.e. strain BB1074, gene rsmF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | - |
Escherichia coli BW25113 | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA | nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA | Escherichia coli BW25113 | S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
housekeeping methyltransferase RsmF | - |
Escherichia coli |
RsmF housekeeping methyltransferase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | DELTArsmF null mutant bacteria have a decreased susceptibility to aminoglycosides, including 4,6- and 4,5-deoxystreptamine aminoglycosides | Escherichia coli |
physiological function | the housekeeping methylation at C1407 is involved in intrinsic aminoglycoside susceptibility in Escherichia coli | Escherichia coli |