BRENDA - Enzyme Database show
show all sequences of 2.1.1.178

YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407

Andersen, N.M.; Douthwaite, S.; J. Mol. Biol. 359, 777-786 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the yebU gene is cloned as a partial and as a fulllength sequence with the respective purposes of creating a knockout strain and expressing recombinant YebU protein. Full-length YebU protein with a C-terminal histidine-tag is expressed in Escherichia coli
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
x * 54000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
Escherichia coli
nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P76273
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria
705111
Escherichia coli
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains
705111
Escherichia coli
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 54000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
the yebU gene is cloned as a partial and as a fulllength sequence with the respective purposes of creating a knockout strain and expressing recombinant YebU protein. Full-length YebU protein with a C-terminal histidine-tag is expressed in Escherichia coli
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
x * 54000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
Escherichia coli
nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria
705111
Escherichia coli
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + cytosine1407 in 16S rRNA
purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains
705111
Escherichia coli
S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
inactivation of the yebU gene leads to loss of methylation at C1407 in 16S rRNA. yebU knockout strains display slower growth and reduced fitness in competition with wild-type cells (at 37°C and 42°C)
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
inactivation of the yebU gene leads to loss of methylation at C1407 in 16S rRNA. yebU knockout strains display slower growth and reduced fitness in competition with wild-type cells (at 37°C and 42°C)
Escherichia coli
Other publictions for EC 2.1.1.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735241
Lioy
Aminoglycoside resistance 16S ...
Escherichia coli, Escherichia coli MM294
RNA
20
382-391
2014
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718596
Gutierrez
Fitness cost and interference ...
Escherichia coli, Escherichia coli BW25113
Antimicrob. Agents Chemother.
56
2335-2341
2012
-
-
1
-
1
-
1
-
-
-
-
2
-
30
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721025
Demirci
Multi-site-specific 16S rRNA m ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
RNA
16
1584-1596
2010
-
-
1
1
1
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
12
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
12
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
706724
Cubrilo
The aminoglycoside resistance ...
Escherichia coli
RNA
15
1492-1497
2009
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705111
Andersen
YebU is a m5C methyltransferas ...
Escherichia coli
J. Mol. Biol.
359
777-786
2006
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
705112
Hallberg
The structure of the RNA m5C m ...
Escherichia coli
J. Mol. Biol.
360
774-787
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
-
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-
-
-
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-
1
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1
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