BRENDA - Enzyme Database show
show all sequences of 2.1.1.177

YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA

Purta, E.; Kaminska, K.H.; Kasprzak, J.M.; Bujnicki, J.M.; Douthwaite, S.; RNA 14, 2234-2244 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0A8I8
-
-
Reaction
Reaction
Commentary
Organism
S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA = S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
mechanism: the enzyme docks into the ribosomal A site without encroaching into the P site. YbeA makes extensive interface contacts with both the 30S and 50S subunits to align its active site cofactor adjacent to nucleotide C1915
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA
-
706719
Escherichia coli
S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA
-
706719
Escherichia coli
S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
-
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
inactivation of the ybeA gene leads to loss of methylation at nucleotide C1915, loss of ybeA function in Escherichia coli causes a slight slowing of the growth rate
Escherichia coli
physiological function
methylation by YbeA possibly functions as a stamp of approval signifying that the 50S subunit has engaged in translational initiation
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
inactivation of the ybeA gene leads to loss of methylation at nucleotide C1915, loss of ybeA function in Escherichia coli causes a slight slowing of the growth rate
Escherichia coli
physiological function
methylation by YbeA possibly functions as a stamp of approval signifying that the 50S subunit has engaged in translational initiation
Escherichia coli
Other publictions for EC 2.1.1.177
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
706718
Ero
Identification of pseudouridin ...
Escherichia coli
RNA
14
2223-2233
2008
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4
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1
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706719
Purta
YbeA is the m3Psi methyltransf ...
Escherichia coli
RNA
14
2234-2244
2008
-
-
1
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1
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5
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1
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1
1
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2
2
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705114
Mallam
A comparison of the folding of ...
Escherichia coli
J. Mol. Biol.
366
650-665
2007
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1
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