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Literature summary for 2.1.1.176 extracted from

  • Demirci, H.; Larsen, L.H.; Hansen, T.; Rasmussen, A.; Cadambi, A.; Gregory, S.T.; Kirpekar, F.; Jogl, G.
    Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus (2010), RNA, 16, 1584-1596.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.176

Cloned(Commentary)

Cloned (Comment) Organism
gene TTHA0851 Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
additional information construction of a null mutant strain in which TTHA0851 is inactivated by homologous recombination and insertion of a heat stable kanamycin-resistance gene Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine967 in 16S rRNA Thermus thermophilus
-
 S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine967 in 16S rRNA Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
 gene TTHA0851 or rsmB
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 gene TTHA0851 or rsmB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate recognition mechanism, overview Thermus thermophilus ?
-
 ?
additional information substrate recognition mechanism, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
S-adenosyl-L-methionine + cytosine967 in 16S rRNA
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
 ?
S-adenosyl-L-methionine + cytosine967 in 16S rRNA
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
 ?

Subunits

Subunits Comment Organism
More comparison of Thermus thermophilus RsmB and RsmF protein sequences, secondary and tertiary structure, differences in substrate binding, overview Thermus thermophilus

Synonyms

Synonyms Comment Organism
16S rRNA methyltransferase
-
 Thermus thermophilus
fmu
-
 Thermus thermophilus
rsmB
-
 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Thermus thermophilus

General Information

General Information Comment Organism
metabolism two methyltransferases, RsmB and RsmF, are responsible for all four 5-methylcytidine modifications in 16S rRNA of Thermus thermophilus, overview. RsmB produces m5C967, while RsmF methylates C1400, C1404, and C1407 in a 30S subunit substrate, but only C1400 and C1404 when naked 16S rRNA is the substrate Thermus thermophilus