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Literature summary for 2.1.1.176 extracted from

  • Tscherne, J.S.; Nurse, K.; Popienick, P.; Michel, H.; Sochacki, M.; Ofengand, J.
    Purification, cloning, and characterization of the 16S RNA m5C967 methyltransferase from Escherichia coli (1999), Biochemistry, 38, 1884-1892.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for added Mg2+, suggesting that extensive secondary or tertiary structure in the RNA substrate may not be a requirement for recognition Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine967 in 16S rRNA Escherichia coli
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S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P36929
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytosine967 in 16S rRNA
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
?
S-adenosyl-L-methionine + cytosine967 in 16S rRNA the recombinant protein exhibits the same specificity as the native enzyme. It forms only m5C and only at position 967. C1407, which is also 5-methylcytosine in natural 16S RNA, is not methylated. In vitro, the enzyme only recognizes free 16S RNA. 30S ribosomal subunits are not a substrate Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA
-
?

Synonyms

Synonyms Comment Organism
rsmB
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli