Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for added Mg2+, suggesting that extensive secondary or tertiary structure in the RNA substrate may not be a requirement for recognition | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine967 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P36929 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine967 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + cytosine967 in 16S rRNA | the recombinant protein exhibits the same specificity as the native enzyme. It forms only m5C and only at position 967. C1407, which is also 5-methylcytosine in natural 16S RNA, is not methylated. In vitro, the enzyme only recognizes free 16S RNA. 30S ribosomal subunits are not a substrate | Escherichia coli | S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rsmB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |