BRENDA - Enzyme Database
show all sequences of 2.1.1.174

Methylated 23S rRNA nucleotide m2G1835 of Escherichia coli ribosome facilitates subunit association

Osterman, I.A.; Sergiev, P.V.; Tsvetkov, P.O.; Makarov, A.A.; Bogdanov, A.A.; Dontsova, O.A.; Biochimie 93, 725-729 (2011)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of rlmG knock-out cells, ribosomes purified from the rlmG knockout strain do not have methyl group attached to nucleotide G1835 of the 23S rRNA
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
Escherichia coli
m2G1835 is located at ribosomal subunits 50S and 30S interface
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
gene rlmG
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
-
719013
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
m2G1835 is located at ribosomal subunits 50S and 30S interface
719013
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of rlmG knock-out cells, ribosomes purified from the rlmG knockout strain do not have methyl group attached to nucleotide G1835 of the 23S rRNA
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
Escherichia coli
m2G1835 is located at ribosomal subunits 50S and 30S interface
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
-
719013
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
m2G1835 is located at ribosomal subunits 50S and 30S interface
719013
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
absence of 23S rRNA nucleotide G1835 methylation does not influence the fidelity of translation or ribosome interaction with translation GTPases and decreases bacterial cell survival at osmotic and oxidative stress. Cells devoid of the rlmG gene are hypersensitive to osmotic and oxidative stress
Escherichia coli
physiological function
methylation of 23S rRNA nucleotide m2G1835 is important for association of ribosomal subunits. Methylation of G1835 provides a significant advantage for bacteria at osmotic and oxidative stress
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
absence of 23S rRNA nucleotide G1835 methylation does not influence the fidelity of translation or ribosome interaction with translation GTPases and decreases bacterial cell survival at osmotic and oxidative stress. Cells devoid of the rlmG gene are hypersensitive to osmotic and oxidative stress
Escherichia coli
physiological function
methylation of 23S rRNA nucleotide m2G1835 is important for association of ribosomal subunits. Methylation of G1835 provides a significant advantage for bacteria at osmotic and oxidative stress
Escherichia coli
Other publictions for EC 2.1.1.174
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721034
Zhang
Structural insights into the f ...
Escherichia coli
RNA
18
1500-1509
2012
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719013
Osterman
Methylated 23S rRNA nucleotide ...
Escherichia coli
Biochimie
93
725-729
2011
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689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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675412
Sergiev
Identification of Escherichia ...
Escherichia coli
J. Mol. Biol.
364
26-31
2006
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