Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of rlmG knock-out cells, ribosomes purified from the rlmG knockout strain do not have methyl group attached to nucleotide G1835 of the 23S rRNA | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1835 in 23S rRNA | Escherichia coli | m2G1835 is located at ribosomal subunits 50S and 30S interface | S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rlmG | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1835 in 23S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine1835 in 23S rRNA | m2G1835 is located at ribosomal subunits 50S and 30S interface | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N2-methyltransferase RlmG | - |
Escherichia coli |
RlmG | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | absence of 23S rRNA nucleotide G1835 methylation does not influence the fidelity of translation or ribosome interaction with translation GTPases and decreases bacterial cell survival at osmotic and oxidative stress. Cells devoid of the rlmG gene are hypersensitive to osmotic and oxidative stress | Escherichia coli |
physiological function | methylation of 23S rRNA nucleotide m2G1835 is important for association of ribosomal subunits. Methylation of G1835 provides a significant advantage for bacteria at osmotic and oxidative stress | Escherichia coli |