BRENDA - Enzyme Database show
show all sequences of 2.1.1.174

Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA

Sergiev, P.V.; Lesnyak, D.V.; Bogdanov, A.A.; Dontsova, O.A.; J. Mol. Biol. 364, 26-31 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant YgjO protein carrying a C-terminal GFP-His6-tag is expressed in Escherichia coli
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71000
-
x * 71000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
Escherichia coli
the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
Q0T0I4
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2
675412
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
recombinant YgjO protein is able to methylate in vitro protein-free 23S rRNA, but not assembled 50S subunits purified from the ygjO knock-out strain
675412
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 71000, SDS-PAGE
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant YgjO protein carrying a C-terminal GFP-His6-tag is expressed in Escherichia coli
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71000
-
x * 71000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
Escherichia coli
the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2
675412
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1835 in 23S rRNA
recombinant YgjO protein is able to methylate in vitro protein-free 23S rRNA, but not assembled 50S subunits purified from the ygjO knock-out strain
675412
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 71000, SDS-PAGE
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
knock-out of the ygjO gene leads to loss of modification at G1835. Lack of the G1835 methylation causes growth retardation, especially at temperatures higher than optimal and in poor media
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
knock-out of the ygjO gene leads to loss of modification at G1835. Lack of the G1835 methylation causes growth retardation, especially at temperatures higher than optimal and in poor media
Escherichia coli
Other publictions for EC 2.1.1.174
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721034
Zhang
Structural insights into the f ...
Escherichia coli
RNA
18
1500-1509
2012
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1
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1
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2
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2
2
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2
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719013
Osterman
Methylated 23S rRNA nucleotide ...
Escherichia coli
Biochimie
93
725-729
2011
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1
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2
2
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689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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1
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675412
Sergiev
Identification of Escherichia ...
Escherichia coli
J. Mol. Biol.
364
26-31
2006
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1
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