Cloned (Comment) | Organism |
---|---|
recombinant YgjO protein carrying a C-terminal GFP-His6-tag is expressed in Escherichia coli | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
x * 71000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1835 in 23S rRNA | Escherichia coli | the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2 | S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q0T0I4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1835 in 23S rRNA | the nucleotide m2G1835 is located in a functionally extremely important region of the ribosome, being located within intersubunit bridges of group B2 | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine1835 in 23S rRNA | recombinant YgjO protein is able to methylate in vitro protein-free 23S rRNA, but not assembled 50S subunits purified from the ygjO knock-out strain | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ribosomal RNA large subunit methyltransferase G | - |
Escherichia coli |
RlmG | - |
Escherichia coli |
ygjO | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | knock-out of the ygjO gene leads to loss of modification at G1835. Lack of the G1835 methylation causes growth retardation, especially at temperatures higher than optimal and in poor media | Escherichia coli |