BRENDA - Reference to 2.1.1.173; Id = 720601

additional information

generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY

Escherichia coli

additional information

Escherichia coli

the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY

?

-

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

Escherichia coli

-

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Escherichia coli

-

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Streptococcus mutans

Smu472 adds the m2G2445 modification

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

Escherichia coli

P75864

gene ycbY

-

Streptococcus mutans

-

additional information

the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY

720601

Escherichia coli

?

-

additional information

Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs

720601

Streptococcus mutans

?

-

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

-

720601

Escherichia coli

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

m7G2069 modification by YcbY is stoichiometric

720601

Escherichia coli

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

-

720601

Streptococcus mutans

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

-

720601

Escherichia coli

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Smu472 adds the m2G2445 modification

720601

Streptococcus mutans

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

m2G2445 modification by YcbY is stoichiometric

720601

Escherichia coli

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

More

structure comparisosns with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview

Escherichia coli

More

structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview

Streptococcus mutans

m2G2445 methyltransferase

-

Escherichia coli

m2G2445 methyltransferase

-

Streptococcus mutans

ribosomal large subunit methyltransferase

-

Escherichia coli

RlmKL

-

Escherichia coli

Smu472

-

Streptococcus mutans

ycbY

-

Escherichia coli

S-adenosyl-L-methionine

-

Escherichia coli

S-adenosyl-L-methionine

-

Streptococcus mutans

S-adenosyl-L-methionine

-

Escherichia coli

S-adenosyl-L-methionine

-

Streptococcus mutans

additional information

generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY

Escherichia coli

additional information

Escherichia coli

the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY

?

-

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

Escherichia coli

-

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Escherichia coli

-

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Streptococcus mutans

Smu472 adds the m2G2445 modification

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

additional information

the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY

720601

Escherichia coli

?

-

additional information

Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs

720601

Streptococcus mutans

?

-

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

-

720601

Escherichia coli

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2069 in 23S rRNA

m7G2069 modification by YcbY is stoichiometric

720601

Escherichia coli

S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

-

720601

Streptococcus mutans

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

-

720601

Escherichia coli

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Smu472 adds the m2G2445 modification

720601

Streptococcus mutans

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

m2G2445 modification by YcbY is stoichiometric

720601

Escherichia coli

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

More

structure comparisosns with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview

Escherichia coli

More

structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview

Streptococcus mutans

evolution

the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered

Escherichia coli

evolution

the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered

Streptococcus mutans

physiological function

the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification

Escherichia coli

evolution

the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered

Escherichia coli

evolution

the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered

Streptococcus mutans

physiological function

the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification

Escherichia coli

720987

Kita

Crystal structure of a putativ ...

Escherichia coli

Protein Pept. Lett.

20

530-537

2013

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

720598

Kimura

Base methylations in the doubl ...

Escherichia coli

Nucleic Acids Res.

40

4071-4085

2012

-

1

-

1

-

1

-

2

-

2

-

4

1

3

1

-

1

-

1

-

1

-

1

-

1

-

2

-

4

1

-

1

-

3

-

720601

Wang

Structure of the bifunctional ...

Escherichia coli, Streptococcus mutans

Nucleic Acids Res.

40

5138-5148

2012

-

1

-

4

-

5

-

8

2

6

-

2

-

2

-

1

-

4

-

8

2

-

3

-

718495

Wang

Purification, crystallization ...

Escherichia coli

Acta Crystallogr. Sect. F

66

1484-1486

2010

-

1

-

2

1

-

3

-

1

-

1

3

-

1

-

1

-

2

1

-

1

-

1

-

689247

Sergiev

Ribosomal RNA guanine-(N2)-met ...

Escherichia coli

Nucleic Acids Res.

35

2295-2301

2007

-

1

-

1

-

2

-

2

-

1

-

2

-

1

-

675411

Lesnyak

Identification of Escherichia ...

Escherichia coli

J. Mol. Biol.

364

20-25

2006

-

1

-

1

-

1

-

1

-

2

1

2

-

1

-

1

-

1

-

2

1

-

2

-

704261

Reddy

Characterization of the uup lo ...

Escherichia coli

J. Bacteriol.

182

1978-1986

2000

-

1

-

1

-

1

-

1

-

1

-

1

-

Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA

Data extracted from this reference: