Data extracted from this reference:
Engineering
additional information
generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY
Escherichia coli
Natural Substrates/ Products (Substrates)
additional information
Escherichia coli
the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Streptococcus mutans
Smu472 adds the m2G2445 modification
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Organism
Escherichia coli
P75864
gene ycbY
Substrates and Products (Substrate)
additional information
the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
720601
Escherichia coli
?
additional information
Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs
720601
Streptococcus mutans
?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
720601
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
m7G2069 modification by YcbY is stoichiometric
720601
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
720601
Streptococcus mutans
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
720601
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Smu472 adds the m2G2445 modification
720601
Streptococcus mutans
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
m2G2445 modification by YcbY is stoichiometric
720601
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Subunits
More
structure comparisosns with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview
Escherichia coli
More
structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview
Streptococcus mutans
Synonyms
m2G2445 methyltransferase
Escherichia coli
m2G2445 methyltransferase
Streptococcus mutans
ribosomal large subunit methyltransferase
Escherichia coli
Smu472
Streptococcus mutans
Cofactor
S-adenosyl-L-methionine
Escherichia coli
S-adenosyl-L-methionine
Streptococcus mutans
Cofactor (protein specific)
S-adenosyl-L-methionine
Escherichia coli
S-adenosyl-L-methionine
Streptococcus mutans
Engineering (protein specific)
additional information
generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
additional information
Escherichia coli
the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Streptococcus mutans
Smu472 adds the m2G2445 modification
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Substrates and Products (Substrate) (protein specific)
additional information
the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
720601
Escherichia coli
?
additional information
Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs
720601
Streptococcus mutans
?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
720601
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
m7G2069 modification by YcbY is stoichiometric
720601
Escherichia coli
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
720601
Streptococcus mutans
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
720601
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Smu472 adds the m2G2445 modification
720601
Streptococcus mutans
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
m2G2445 modification by YcbY is stoichiometric
720601
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Subunits (protein specific)
More
structure comparisosns with Streptococcus mutans proteins Smu472 and Smu776, the active site and their folding patterns respectively resemble each other, overview
Escherichia coli
More
structure comparisosns of Streptococcus mutans proteins Smu472 and Smu776 with Escherichia coli RlmKL protein, the active site and their folding patterns respectively resemble each other, overview
Streptococcus mutans
General Information
evolution
the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
Escherichia coli
evolution
the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
Streptococcus mutans
physiological function
the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification
Escherichia coli
General Information (protein specific)
evolution
the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
Escherichia coli
evolution
the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
Streptococcus mutans
physiological function
the N-terminal region of YcbY adds the m2G2445 modification, while the C-terminal region of YcbY is responsible for the m7G2069 methylation on the opposite side of the same helix, H74. YcbY enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification
Escherichia coli
Other publictions for EC 2.1.1.173
720987
Kita
Crystal structure of a putativ ...
Escherichia coli
Protein Pept. Lett.
20
530-537
2013
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720598
Kimura
Base methylations in the doubl ...
Escherichia coli
Nucleic Acids Res.
40
4071-4085
2012
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720601
Wang
Structure of the bifunctional ...
Escherichia coli, Streptococcus mutans
Nucleic Acids Res.
40
5138-5148
2012
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718495
Wang
Purification, crystallization ...
Escherichia coli
Acta Crystallogr. Sect. F
66
1484-1486
2010
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689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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675411
Lesnyak
Identification of Escherichia ...
Escherichia coli
J. Mol. Biol.
364
20-25
2006
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704261
Reddy
Characterization of the uup lo ...
Escherichia coli
J. Bacteriol.
182
1978-1986
2000
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