Data extracted from this reference:
Cloned(Commentary)
gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)
Escherichia coli
Crystallization (Commentary)
purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution
Escherichia coli
Molecular Weight [Da]
78900
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
Escherichia coli
82000
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Organism
Escherichia coli
P75864
gene rlmL, previously known as ycbY
Purification (Commentary)
recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
Escherichia coli
Substrates and Products (Substrate)
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
718495
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Subunits
?
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
Escherichia coli
Synonyms
23S RNA m(2)G2445 methyltransferase
Escherichia coli
Cofactor
S-adenosyl-L-methionine
dependent on
Escherichia coli
Cloned(Commentary) (protein specific)
gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)
Escherichia coli
Cofactor (protein specific)
S-adenosyl-L-methionine
dependent on
Escherichia coli
Crystallization (Commentary) (protein specific)
purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution
Escherichia coli
Molecular Weight [Da] (protein specific)
78900
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
Escherichia coli
82000
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Purification (Commentary) (protein specific)
recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
718495
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
?
Subunits (protein specific)
?
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
Escherichia coli
Other publictions for EC
720987
Kita
Crystal structure of a putativ ...
Escherichia coli
Protein Pept. Lett.
20
530-537
2013
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720598
Kimura
Base methylations in the doubl ...
Escherichia coli
Nucleic Acids Res.
40
4071-4085
2012
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3
3
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720601
Wang
Structure of the bifunctional ...
Escherichia coli, Streptococcus mutans
Nucleic Acids Res.
40
5138-5148
2012
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3
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718495
Wang
Purification, crystallization ...
Escherichia coli
Acta Crystallogr. Sect. F
66
1484-1486
2010
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1
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689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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675411
Lesnyak
Identification of Escherichia ...
Escherichia coli
J. Mol. Biol.
364
20-25
2006
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704261
Reddy
Characterization of the uup lo ...
Escherichia coli
J. Bacteriol.
182
1978-1986
2000
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