BRENDA - Reference to 2.1.1.173; Id = 718495

gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)

Escherichia coli

purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution

Escherichia coli

78900

-

x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE

Escherichia coli

82000

-

x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE

Escherichia coli

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Escherichia coli

-

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

Escherichia coli

P75864

gene rlmL, previously known as ycbY

-

recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity

Escherichia coli

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

-

718495

Escherichia coli

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE

Escherichia coli

23S RNA m(2)G2445 methyltransferase

-

Escherichia coli

RlmL

-

Escherichia coli

ycbY

-

Escherichia coli

S-adenosyl-L-methionine

dependent on

Escherichia coli

gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)

Escherichia coli

S-adenosyl-L-methionine

dependent on

Escherichia coli

purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution

Escherichia coli

78900

-

x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE

Escherichia coli

82000

-

x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE

Escherichia coli

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

Escherichia coli

-

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity

Escherichia coli

S-adenosyl-L-methionine + guanine2445 in 23S rRNA

-

718495

Escherichia coli

S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA

-

?

x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE

Escherichia coli

720987

Kita

Crystal structure of a putativ ...

Escherichia coli

Protein Pept. Lett.

20

530-537

2013

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

720598

Kimura

Base methylations in the doubl ...

Escherichia coli

Nucleic Acids Res.

40

4071-4085

2012

-

1

-

1

-

1

-

2

-

2

-

4

1

3

1

-

1

-

1

-

1

-

1

-

1

-

2

-

4

1

-

1

-

3

-

720601

Wang

Structure of the bifunctional ...

Escherichia coli, Streptococcus mutans

Nucleic Acids Res.

40

5138-5148

2012

-

1

-

4

-

5

-

8

2

6

-

2

-

2

-

1

-

4

-

8

2

-

3

-

718495

Wang

Purification, crystallization ...

Escherichia coli

Acta Crystallogr. Sect. F

66

1484-1486

2010

-

1

-

2

1

-

3

-

1

-

1

3

-

1

-

1

-

2

1

-

1

-

1

-

689247

Sergiev

Ribosomal RNA guanine-(N2)-met ...

Escherichia coli

Nucleic Acids Res.

35

2295-2301

2007

-

1

-

1

-

2

-

2

-

1

-

2

-

1

-

675411

Lesnyak

Identification of Escherichia ...

Escherichia coli

J. Mol. Biol.

364

20-25

2006

-

1

-

1

-

1

-

1

-

2

1

2

-

1

-

1

-

1

-

2

1

-

2

-

704261

Reddy

Characterization of the uup lo ...

Escherichia coli

J. Bacteriol.

182

1978-1986

2000

-

1

-

1

-

1

-

1

-

1

-

1

-

Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli

Data extracted from this reference: