BRENDA - Enzyme Database show
show all sequences of 2.1.1.172

Functional specialization of domains tandemly duplicated within 16S rRNA methyltransferase RsmC

Sunita, S.; Purta, E.; Durawa, M.; Tkaczuk, K.L.; Swaathi, J.; Bujnicki, J.M.; Sivaraman, J.; Nucleic Acids Res. 35, 4264-4274 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the rsmC gene, cloned into pCA24N vector with a noncleavable N-terminal His6 tag
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, crystal structure of RsmC refined to 2.1 A resolution, reveals two homologous domains tandemly duplicated within a single polypeptide. Characterization of the function of the individual domains and identification of key residues involved in binding of rRNA and S-adenosyl-L-methionine, and in catalysis. It is discovered that one of the domains is important for the folding of the other. RsmC can be regarded as a model system for functional streamlining of domains accompanied by the development of dependencies concerning folding and stability
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P39406
-
-
Purification (Commentary)
Commentary
Organism
purification and refolding of C-RsmC from inclusion bodies
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
-
705959
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
the rsmC gene, cloned into pCA24N vector with a noncleavable N-terminal His6 tag
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, crystal structure of RsmC refined to 2.1 A resolution, reveals two homologous domains tandemly duplicated within a single polypeptide. Characterization of the function of the individual domains and identification of key residues involved in binding of rRNA and S-adenosyl-L-methionine, and in catalysis. It is discovered that one of the domains is important for the folding of the other. RsmC can be regarded as a model system for functional streamlining of domains accompanied by the development of dependencies concerning folding and stability
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
purification and refolding of C-RsmC from inclusion bodies
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
-
705959
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
-
?
Other publictions for EC 2.1.1.172
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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705959
Sunita
Functional specialization of d ...
Escherichia coli
Nucleic Acids Res.
35
4264-4274
2007
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702877
Bujnicki
Rychlewski. L.: RNA:(guanine-N ...
Escherichia coli
BMC Bioinformatics
3
0000
2002
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1
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440040
Bujnicki
Phylogenomic analysis of 16S r ...
Escherichia coli
FASEB J.
14
2365-2368
2000
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440041
Tscherne
Purification, cloning, and cha ...
Escherichia coli
J. Biol. Chem.
274
924-929
1999
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1
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2
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1
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5
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