BRENDA - Enzyme Database show
show all sequences of 2.1.1.172

Purification, cloning, and characterization of the 16S RNA m2G1207 methyltransferase from Escherichia coli

Tscherne, J.S.; Nurse, K.; Popienick, P.; Ofengand, J.; J. Biol. Chem. 274, 924-929 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
2 mM, markedly reduces the level of methylation
Escherichia coli
Mg2+
6 mM, markedly reduces the level of methylation
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
close to unit stoichiometry of methylation can be achieved at 0.9 mM Mg2+
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
gel filtration
Escherichia coli
37600
-
1 * 37600, calculated from sequence
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
Escherichia coli
because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P39406
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins
440041
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
the enzyme reacts well with 30S subunits reconstituted from 16S RNA transcripts and 30S proteins but is almost inactive with the corresponding free RNA. Of the three naturally occurring N2-methylguanine residues, only N2-methylguanine1207 is formed. It is suggested that the optimal substrate may be a ribonucleoprotein particle less structured than a 30S ribosome but more so than free RNA. Localization of the site of methylation by hybridization-protection studies using deoxyoligomers that are complementary to the RNA sequence spanning each of the N2-methylguanine sites
440041
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 37600, calculated from sequence
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
2 mM, markedly reduces the level of methylation
Escherichia coli
Mg2+
6 mM, markedly reduces the level of methylation
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
close to unit stoichiometry of methylation can be achieved at 0.9 mM Mg2+
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
gel filtration
Escherichia coli
37600
-
1 * 37600, calculated from sequence
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
Escherichia coli
because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins
440041
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1207 in 16S rRNA
the enzyme reacts well with 30S subunits reconstituted from 16S RNA transcripts and 30S proteins but is almost inactive with the corresponding free RNA. Of the three naturally occurring N2-methylguanine residues, only N2-methylguanine1207 is formed. It is suggested that the optimal substrate may be a ribonucleoprotein particle less structured than a 30S ribosome but more so than free RNA. Localization of the site of methylation by hybridization-protection studies using deoxyoligomers that are complementary to the RNA sequence spanning each of the N2-methylguanine sites
440041
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37600, calculated from sequence
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
Other publictions for EC 2.1.1.172
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705959
Sunita
Functional specialization of d ...
Escherichia coli
Nucleic Acids Res.
35
4264-4274
2007
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702877
Bujnicki
Rychlewski. L.: RNA:(guanine-N ...
Escherichia coli
BMC Bioinformatics
3
0000
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440040
Bujnicki
Phylogenomic analysis of 16S r ...
Escherichia coli
FASEB J.
14
2365-2368
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440041
Tscherne
Purification, cloning, and cha ...
Escherichia coli
J. Biol. Chem.
274
924-929
1999
-
-
1
-
-
-
2
-
-
1
2
1
-
5
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-