Protein Variants | Comment | Organism |
---|---|---|
DeltaN30 | truncated PmtA variant missing the first 30 amino acids, completely inactive | Agrobacterium tumefaciens |
F19A | inactive | Agrobacterium tumefaciens |
K6Q/R8Q/K12Q | mutant produced wild-type-like amounts of the first intermediate monomethyl-phosphatidylethanolamine (C18:1) and reduced amounts of dimethyl-phosphatidylethanolamine (C18:1) and phosphatidylcholine | Agrobacterium tumefaciens |
R18Q/K21Q/K28Q/K29Q | mutations of the positively charged residues in the C-terminal part of the alphaA helix, do not influence polar localization | Agrobacterium tumefaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme attaches to the membrane via electrostatic interactions with anionic lipids, which do not serve as substrate. Increasing phosphatidylcholine concentrations trigger membrane dissociation suggesting that membrane binding of PmtA is negatively regulated by its end product phosphatidylcholine. alpha-Helical regions alphaA and alphaF contribute to membrane binding. The latter undergoes a structural transition from disordered to alpha-helical conformation in the presence of anionic lipids. The basic amino acids R8 and K12 and the hydrophobic amino acid F19 are critical for membrane binding by alphaA as well as for activity of full-length PmtA | Agrobacterium tumefaciens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
PmtA | - |
Agrobacterium tumefaciens |