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Literature summary for 2.1.1.17 extracted from

  • Kharbanda, K.K.; Mailliard, M.E.; Baldwin, C.R.; Beckenhauer, H.C.; Sorrell, M.F.; Tuma, D.J.
    Betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway (2007), J. Hepatol., 46, 314-321.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, the effect is enhanced by ethanol, which alone has no effect on expression levels of the enzyme, overview Rattus norvegicus
ethanol enhances the inducing and protecting effects of betaine on the enzyme, alone has only a slightly activating effect on the enzyme activity, overview Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + phosphatidylethanolamine Rattus norvegicus betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview 3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
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?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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male Wistar rats weighing 180-200 g. 30-40% less phosphatidylcholine is generated in hepatocytes from ethanol-fed rats as compared to controls. Hepatocytes isolated from rats fed the betaine-supplemented ethanol diet have significantly higher phosphatidylcholine generation compared to hepatocytes from ethanol-fed rats.
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Rattus norvegicus Q08388 male Wistar rats
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Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte
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Rattus norvegicus
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liver
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Rattus norvegicus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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there is a significant positive linear relationship between phosphatidylethanolamine N-methyltransferase activity and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = 0.45). There is a significant inverse relationship between phosphatidylethanolamine N-methyltransferase activity and triglycerids. The metabolic conversion of radiolabeled phosphatidylethanolamine to phosphatidylcholine over 3 h incubation period is taken as a measure of phosphatidylethanolamine N-methyltransferase. Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + phosphatidylethanolamine
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Rattus norvegicus 3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
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?
3 S-adenosyl-L-methionine + phosphatidylethanolamine betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview Rattus norvegicus 3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+
-
?
additional information the total hepatic S-adenosylmethionine levels remain unchanged by ethanol consumption for 4 weeks Rattus norvegicus additional information S-adenosylhomocysteine levels increase by ethanol consumption for 4 weeks. The S-adenosylmethionine:S-adenosylhomocysteine ratios are significantly lower in ethanol fed rats. Supplementation of betaine in the ethanol diet increases the hepatocellular S-adenosylmethionine:S-adenosylhomocysteine ratios to values that are statistically similar to those from rats fed the regular control diet. There is an significant inverse relationship between triglycerids and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = - 0.36). ?

Synonyms

Synonyms Comment Organism
PEMT
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Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
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assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Rattus norvegicus