Activating Compound | Comment | Organism | Structure |
---|---|---|---|
betaine | attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, the effect is enhanced by ethanol, which alone has no effect on expression levels of the enzyme, overview | Rattus norvegicus | |
ethanol | enhances the inducing and protecting effects of betaine on the enzyme, alone has only a slightly activating effect on the enzyme activity, overview | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + phosphatidylethanolamine | Rattus norvegicus | betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview | 3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats weighing 180-200 g. 30-40% less phosphatidylcholine is generated in hepatocytes from ethanol-fed rats as compared to controls. Hepatocytes isolated from rats fed the betaine-supplemented ethanol diet have significantly higher phosphatidylcholine generation compared to hepatocytes from ethanol-fed rats. | - |
Rattus norvegicus | Q08388 | male Wistar rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
there is a significant positive linear relationship between phosphatidylethanolamine N-methyltransferase activity and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = 0.45). There is a significant inverse relationship between phosphatidylethanolamine N-methyltransferase activity and triglycerids. The metabolic conversion of radiolabeled phosphatidylethanolamine to phosphatidylcholine over 3 h incubation period is taken as a measure of phosphatidylethanolamine N-methyltransferase. | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + phosphatidylethanolamine | - |
Rattus norvegicus | 3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+ | - |
? | |
3 S-adenosyl-L-methionine + phosphatidylethanolamine | betaine attenuates alcoholic steatosis by restoring phosphatidylcholine generation via the phosphatidylethanolamine methyltransferase pathway, overview | Rattus norvegicus | 3 S-adenosyl-L-homocysteine + phosphatidylcholine + 2 H+ | - |
? | |
additional information | the total hepatic S-adenosylmethionine levels remain unchanged by ethanol consumption for 4 weeks | Rattus norvegicus | additional information | S-adenosylhomocysteine levels increase by ethanol consumption for 4 weeks. The S-adenosylmethionine:S-adenosylhomocysteine ratios are significantly lower in ethanol fed rats. Supplementation of betaine in the ethanol diet increases the hepatocellular S-adenosylmethionine:S-adenosylhomocysteine ratios to values that are statistically similar to those from rats fed the regular control diet. There is an significant inverse relationship between triglycerids and S-adenosylmethionine:S-adenosylhomocysteine ratio (r = - 0.36). | ? |
Synonyms | Comment | Organism |
---|---|---|
PEMT | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Rattus norvegicus |