BRENDA - Enzyme Database show
show all sequences of 2.1.1.169

Biochemical characterization of a putative wheat caffeic acid O-methyltransferase

Zhou, J.M.; Seo, Y.W.; Ibrahim, R.K.; Plant Physiol. Biochem. 47, 322-326 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Triticum aestivum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for Mg2+
Triticum aestivum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38900
-
x * 38900, calculated from sequence, SDS-PAGE
Triticum aestivum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Triticum aestivum
Q84N28
-
-
Purification (Commentary)
Commentary
Organism
-
Triticum aestivum
Storage Stability
Storage Stability
Organism
4C, almost no loss of catalytic activity when stored for 3-4 weeks
Triticum aestivum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3 S-adenosyl-L-methionine + tricetin
TaOMT1 (TaCOMT1) catalyzes the sequential methylation of tricetin to its 3'-O-monomethyl-derivative (selgin), 3',5'-O-dimethyl-derivative (tricin) and 3',4',5'-O-trimethyl derivative. 3',5'-O-dimethyltricetin appears to be the major reaction product. The enzyme also catalyzes methylation of luteolin, quercetin and eriodictyol
706268
Triticum aestivum
3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
-
-
-
?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
-
706268
Triticum aestivum
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methyltricetin
-
706268
Triticum aestivum
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
-
-
?
S-adenosyl-L-methionine + tricetin
-
706268
Triticum aestivum
S-adenosyl-L-homocysteine + 3'-O-methyltricetin
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38900, calculated from sequence, SDS-PAGE
Triticum aestivum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Triticum aestivum
calculated from sequence
-
5.75
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Triticum aestivum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for Mg2+
Triticum aestivum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38900
-
x * 38900, calculated from sequence, SDS-PAGE
Triticum aestivum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Triticum aestivum
Storage Stability (protein specific)
Storage Stability
Organism
4C, almost no loss of catalytic activity when stored for 3-4 weeks
Triticum aestivum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3 S-adenosyl-L-methionine + tricetin
TaOMT1 (TaCOMT1) catalyzes the sequential methylation of tricetin to its 3'-O-monomethyl-derivative (selgin), 3',5'-O-dimethyl-derivative (tricin) and 3',4',5'-O-trimethyl derivative. 3',5'-O-dimethyltricetin appears to be the major reaction product. The enzyme also catalyzes methylation of luteolin, quercetin and eriodictyol
706268
Triticum aestivum
3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
-
-
-
?
S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin
-
706268
Triticum aestivum
S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin
-
-
-
?
S-adenosyl-L-methionine + 3'-O-methyltricetin
-
706268
Triticum aestivum
S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
-
-
?
S-adenosyl-L-methionine + tricetin
-
706268
Triticum aestivum
S-adenosyl-L-homocysteine + 3'-O-methyltricetin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38900, calculated from sequence, SDS-PAGE
Triticum aestivum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Triticum aestivum
calculated from sequence
-
5.75
Other publictions for EC 2.1.1.169
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734977
Moheb
Tricin biosynthesis during gro ...
Triticum aestivum
Plant Sci.
201-202
115-120
2013
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2
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2
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2
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-
719183
Zhou
Structure-function relationshi ...
Triticum aestivum
BMC Plant Biol.
10
156
2010
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-
1
-
16
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6
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4
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3
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1
4
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6
2
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1
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1
1
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16
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6
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4
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1
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6
2
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2
2
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6
6
703623
Kornblatt
Structure-activity relationshi ...
Triticum aestivum
FEBS J.
275
2255-2266
2008
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1
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1
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1
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4
2
1
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1
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1
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4
2
1
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706268
Zhou
Biochemical characterization o ...
Triticum aestivum
Plant Physiol. Biochem.
47
322-326
2008
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1
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1
1
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2
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1
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1
4
1
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1
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1
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1
1
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1
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1
4
1
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1
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702400
Zhou
Sequential O-methylation of tr ...
Triticum aestivum
Biochim. Biophys. Acta
1760
1115-1124
2006
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1
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3
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1
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1
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1
4
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1
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1
1
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1
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1
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1
4
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