Literature summary for 2.1.1.166 extracted from

Caldas, T.; Binet, E.; Bouloc, P.; Costa, A.; Desgres, J.; Tanaka, Y.; Tokuyama, S.; Ochi, K.; Richarme, G.
The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase (2000), J. Biol. Chem., 275, 16414-16419.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	A0A140N5X9	-	-

Purification (Commentary)

Purification (Comment)	Organism
a chimera protein of RrmJ fused to a chitin-binding domain was purified by affinity chromatography on a chitin affinity column	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + uridine2552 in 23S rRNA	identification of the methylated nucleotide as 2'-O-methyluridine 2552, by reverse phase high performance liquid chromatography analysis, boronate affinity chromatography, and hybridization-protection experiments. In vitro, FtsJ does not efficiently methylate ribosomes purified from a strain producing FtsJ, suggesting that these ribosomes are already methylated in vivo by FtsJ. FtsJ is active on ribosomes and on the 50 S ribosomal subunit, but is inactive on free rRNA, suggesting that its natural substrate is ribosomes or a pre-ribosomal ribonucleoprotein particle	Escherichia coli	S-adenosyl-L-homocysteine + 2'-O-methyluridine2552 in 23S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
23 S ribosomal RNA methyltransferase	-	Escherichia coli
FTSJ	-	Escherichia coli
FtsJ/RrmJ heat shock protein	-	Escherichia coli
RrmJ	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli

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Release 2023.1 (January 2023)