BRENDA - Enzyme Database show
show all sequences of 2.1.1.165

Characterization of three halide methyltransferases in Arabidopsis thaliana

Nagatoshi. Y.; Nakamura, T.; Plant Biotechnol. 24, 503-506 (2007)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53300
-
x * 53300, SDS-PAGE
Arabidopsis thaliana
53500
-
x * 53500, SDS-PAGE; x * 53500, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Arabidopsis thaliana
the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O80562
-
-
Arabidopsis thaliana
Q0WP12
-
-
Arabidopsis thaliana
Q6AWU6
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000058
-
production of methyl iodide
Arabidopsis thaliana
0.00009
-
production of methyl iodide
Arabidopsis thaliana
205
-
production of methyl iodide
Arabidopsis thaliana
Storage Stability
Storage Stability
Organism
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity
Arabidopsis thaliana
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity
Arabidopsis thaliana
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis
700702
Arabidopsis thaliana
?
-
-
-
-
S-adenosyl-L-methionine + chloride
-
700702
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl chloride
-
-
-
?
S-adenosyl-L-methionine + iodide
recombinant protein methylates iodide with greater efficiency than chloride
700702
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl iodide
-
-
-
?
S-adenosyl-L-methionine + iodide
recombinant proteins methylate iodide with greater efficiency than chloride
700702
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl iodide
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 53300, SDS-PAGE; x * 53500, SDS-PAGE; x * 53500, SDS-PAGE
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53300
-
x * 53300, SDS-PAGE
Arabidopsis thaliana
53500
-
x * 53500, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Arabidopsis thaliana
the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000058
-
production of methyl iodide
Arabidopsis thaliana
0.00009
-
production of methyl iodide
Arabidopsis thaliana
205
-
production of methyl iodide
Arabidopsis thaliana
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity
Arabidopsis thaliana
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity
Arabidopsis thaliana
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis
700702
Arabidopsis thaliana
?
-
-
-
-
S-adenosyl-L-methionine + chloride
-
700702
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl chloride
-
-
-
?
S-adenosyl-L-methionine + iodide
recombinant protein methylates iodide with greater efficiency than chloride
700702
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl iodide
-
-
-
?
S-adenosyl-L-methionine + iodide
recombinant proteins methylate iodide with greater efficiency than chloride
700702
Arabidopsis thaliana
S-adenosyl-L-homocysteine + methyl iodide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53500, SDS-PAGE
Arabidopsis thaliana
?
x * 53300, SDS-PAGE
Arabidopsis thaliana
Other publictions for EC 2.1.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696997
Itoh
Involvement of S-adenosylmethi ...
Raphanus sativus
BMC Plant Biol.
9
116
2009
-
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1
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4
-
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2
1
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1
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1
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1
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5
1
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1
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2
1
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1
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1
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5
1
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-
698496
Bayer
Synthesis of methyl halides fr ...
Batis maritima, Brassica rapa, Burkholderia pseudomallei, Burkholderia thailandensis, Oryza sativa, Paraburkholderia phytofirmans, Paraburkholderia xenovorans, Vitis vinifera
J. Am. Chem. Soc.
131
6508-6515
2009
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8
8
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16
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21
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8
8
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21
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698916
Nagatoshi Y
Arabidopsis HARMLESS TO OZONE ...
Arabidopsis thaliana
J. Biol. Chem.
284
19301-19309
2009
-
-
-
-
-
-
-
-
-
-
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1
-
3
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1
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1
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1
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700702
Nagatoshi. Y.; Nakamura
-
Characterization of three hali ...
Arabidopsis thaliana
Plant Biotechnol.
24
503-506
2007
-
-
1
-
-
-
-
-
-
-
2
3
-
3
-
-
-
-
-
-
3
3
9
1
-
-
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-
-
-
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-
-
-
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-
-
3
-
-
-
-
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-
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3
3
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3
3
9
3
-
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-
-
679357
Rhew
Genetic control of methyl hali ...
Arabidopsis thaliana
Curr. Biol.
13
1809-1813
2003
-
-
1
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1
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1
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1
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1
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1
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1
-
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-
-
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-
-
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-
-
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-
695730
Amachi
Bacteria mediate methylation o ...
Rhizobium sp., Rhizobium sp. MRCD 19
Appl. Environ. Microbiol.
67
2718-2722
2001
-
-
-
-
-
-
1
2
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2
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2
-
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1
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4
-
1
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1
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1
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2
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2
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1
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4
-
1
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1
-
-
-
-
-
-
-
-
-
696802
Ohsawa
Purification and characterizat ...
Pavlova pinguis
Biosci. Biotechnol. Biochem.
65
2397-2404
2001
-
-
-
-
-
-
14
2
-
1
1
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-
2
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1
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1
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4
1
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-
1
1
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1
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-
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-
14
-
2
-
1
1
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-
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1
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-
1
-
4
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
682595
Ni
Expression of Batis maritima m ...
Batis maritima
Proc. Natl. Acad. Sci. USA
96
3611-3615
1999
1
-
1
-
-
-
1
8
-
1
-
1
-
5
-
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-
-
-
-
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4
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3
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1
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1
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1
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8
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1
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1
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4
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3
-
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-
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-
677650
Saxena
Biochemical characterization o ...
Phellinus pomaceus
Appl. Environ. Microbiol.
64
2831-2835
1998
-
-
-
-
-
-
1
4
1
-
-
1
-
3
-
-
1
-
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1
-
1
4
-
-
-
-
-
1
1
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1
-
-
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1
-
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1
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4
1
-
-
1
-
-
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1
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1
-
1
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
682593
Ni
cDNA cloning of Batis maritima ...
Batis maritima
Proc. Natl. Acad. Sci. USA
95
12866-12871
1998
-
-
1
-
1
-
-
-
-
-
3
1
-
3
-
-
1
-
-
1
1
1
4
1
1
-
-
-
1
-
-
1
-
1
-
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-
1
1
-
1
-
-
-
-
-
-
-
3
1
-
-
-
1
-
1
1
1
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
700660
Itoh
-
Formation and emission of mono ...
Papenfussiella kuromo, Pavlova gyrans, Sargassum horneri
Phytochemistry
45
67-73
1997
-
-
-
-
-
-
1
2
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3
-
3
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4
8
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1
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1
1
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1
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2
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3
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4
8
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
680600
Attieh
Purification and characterizat ...
Brassica oleracea
J. Biol. Chem.
270
9250-9257
1995
-
-
-
-
-
-
2
4
-
-
2
-
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2
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1
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1
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4
4
1
1
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1
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2
1
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2
2
4
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2
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1
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1
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4
4
1
1
-
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-
1
-
-
1
-
-
-
-
-
-
700704
Saini
-
Biosynthesis of halomethanes a ...
Brassica oleracea
Plant Cell Environ.
18
1027-1033
1995
-
-
-
-
-
-
-
-
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1
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1
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4
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1
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4
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-
701215
Wuosmaa
Methyl chloride transferase: a ...
Endocladia muricata
Science
249
160-162
1990
-
-
-
-
-
-
-
2
-
-
1
-
-
4
-
-
1
-
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1
-
4
-
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1
1
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2
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1
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1
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1
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4
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1
1
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