Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
gene heps-2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109 | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression in Escherichia coli complements mutation AN70 deficient of a specific methyltransferase in the ubiquinone biosynthetic pathway | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | O86169 | - |
- |
Geobacillus stearothermophilus | O86169 | gene heps-2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-heptaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine | - |
Geobacillus stearothermophilus | 2-heptaprenyl-6-methoxy-3-methyl-1,4-benzoquinone + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-heptaprenyl-1,4-naphthoquinone methyltransferase | - |
Geobacillus stearothermophilus |
menaquinone biosynthesis methyltransferase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Geobacillus stearothermophilus |
General Information | Comment | Organism |
---|---|---|
evolution | ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes | Geobacillus stearothermophilus |
metabolism | the demethylmenaquinone methyltransferase or 2-heptaprenyl-1,4-naphthoquinone synthase participates in the terminal step of the menaquinone biosynthesis | Geobacillus stearothermophilus |