BRENDA - Enzyme Database show
show all sequences of 2.1.1.163

Identification of a novel gene cluster participating in menaquinone (vitamin K2) biosynthesis. Cloning and sequence determination of the 2-heptaprenyl-1,4-naphthoquinone methyltransferase gene of Bacillus stearothermophilus

Koike-Takeshita, A.; Koyama, T.; Ogura, K.; J. Biol. Chem. 272, 12380-12383 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; gene heps-2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109
Geobacillus stearothermophilus
Engineering
Amino acid exchange
Commentary
Organism
additional information
expression in Escherichia coli complements mutation AN70 deficient of a specific methyltransferase in the ubiquinone biosynthetic pathway
Geobacillus stearothermophilus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Geobacillus stearothermophilus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Geobacillus stearothermophilus
O86169
; gene heps-2
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-heptaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
-
698706
Geobacillus stearothermophilus
2-heptaprenyl-6-methoxy-3-methyl-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Geobacillus stearothermophilus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Geobacillus stearothermophilus
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
dependent on
Geobacillus stearothermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; gene heps-2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109
Geobacillus stearothermophilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
dependent on
Geobacillus stearothermophilus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
expression in Escherichia coli complements mutation AN70 deficient of a specific methyltransferase in the ubiquinone biosynthetic pathway
Geobacillus stearothermophilus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Geobacillus stearothermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-heptaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
-
698706
Geobacillus stearothermophilus
2-heptaprenyl-6-methoxy-3-methyl-1,4-benzoquinone + S-adenosyl-L-homocysteine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Geobacillus stearothermophilus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Geobacillus stearothermophilus
General Information
General Information
Commentary
Organism
evolution
ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes
Geobacillus stearothermophilus
metabolism
the demethylmenaquinone methyltransferase or 2-heptaprenyl-1,4-naphthoquinone synthase participates in the terminal step of the menaquinone biosynthesis
Geobacillus stearothermophilus
General Information (protein specific)
General Information
Commentary
Organism
evolution
ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes
Geobacillus stearothermophilus
metabolism
the demethylmenaquinone methyltransferase or 2-heptaprenyl-1,4-naphthoquinone synthase participates in the terminal step of the menaquinone biosynthesis
Geobacillus stearothermophilus
Other publictions for EC 2.1.1.163
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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695912
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1
1
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698549
Lee
A C-methyltransferase involved ...
Escherichia coli
J. Bacteriol.
179
1748-1754
1997
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1
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2
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1
1
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698706
Koike-Takeshita
Identification of a novel gene ...
Geobacillus stearothermophilus
J. Biol. Chem.
272
12380-12383
1997
-
-
1
-
1
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-
-
1
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5
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2
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695910
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An Escherichia coli mutant con ...
Escherichia coli, Escherichia coli AN387
Arch. Microbiol.
158
68-73
1992
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1
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4
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1
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4
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3
3
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