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show all sequences of 2.1.1.163

A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene

Lee, P.T.; Hsu, A.Y.; Ha, H.T.; Clarke, C.F.; J. Bacteriol. 179, 1748-1754 (1997)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
G142D
mutant accumulates 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0A887
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-methoxy-2-octaprenyl-1,4-benzoquinol + S-adenosyl-L-methionine
-
698549
Escherichia coli
6-methoxy-3-methyl-2-octaprenyl-1,4-benzoquinol + S-adenosyl-L-homocysteine
-
-
-
?
demethylmenaquinol + S-adenosyl-L-methionine
-
698549
Escherichia coli
menaquinol + S-adenosyl-L-homocysteine
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
G142D
mutant accumulates 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-methoxy-2-octaprenyl-1,4-benzoquinol + S-adenosyl-L-methionine
-
698549
Escherichia coli
6-methoxy-3-methyl-2-octaprenyl-1,4-benzoquinol + S-adenosyl-L-homocysteine
-
-
-
?
demethylmenaquinol + S-adenosyl-L-methionine
-
698549
Escherichia coli
menaquinol + S-adenosyl-L-homocysteine
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
strains containing either a disruption or point mutation G142D in ubiE accumulate 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates. Disruption mutants show defects in growth on succinate. The UbiE polypeptide is required for the C methylation reactions in both ubiquinone and menaquinone biosynthesis
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
strains containing either a disruption or point mutation G142D in ubiE accumulate 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates. Disruption mutants show defects in growth on succinate. The UbiE polypeptide is required for the C methylation reactions in both ubiquinone and menaquinone biosynthesis
Escherichia coli
Other publictions for EC 2.1.1.163
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720846
Xie
Identification of a novel gene ...
Mesorhizobium huakuii, Mesorhizobium huakuii 7653R, no activity in Rhizobium sp.
PLoS ONE
6
e28995
2011
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7
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2
2
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700837
Lee
Trapping and characterization ...
Oryza sativa
Plant Sci.
166
69-79
2004
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695912
Tyson
Characterisation of Escherichi ...
Escherichia coli
Arch. Microbiol.
168
403-411
1997
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1
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698549
Lee
A C-methyltransferase involved ...
Escherichia coli
J. Bacteriol.
179
1748-1754
1997
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1
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2
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1
1
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698706
Koike-Takeshita
Identification of a novel gene ...
Geobacillus stearothermophilus
J. Biol. Chem.
272
12380-12383
1997
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1
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1
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1
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5
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2
2
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695910
Wissenbach
An Escherichia coli mutant con ...
Escherichia coli, Escherichia coli AN387
Arch. Microbiol.
158
68-73
1992
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