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Literature summary for 2.1.1.162 extracted from

  • Kageyama, H.; Tanaka, Y.; Takabe, T.
    Biosynthetic pathways of glycinebetaine in Thalassiosira pseudonana; functional characterization of enzyme catalyzing three-step methylation of glycine (2018), Plant Physiol. Biochem., 127, 248-255 .
    View publication on PubMed
Search for more literature for 2.1.1.162

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thalassiosira pseudonana

Protein Variants

Protein Variants Comment Organism
additional information GSDMT protein has two repeated AdoMet-binding motifs in the N-terminal and C-terminal domains. A truncation mutant carrying the N-terminal part exhibits high activity for glycine, low activity for sarcosine and no activity for dimethylglycine. A truncation mutant carrying the C-terminal part displays the highest activity to dimethylgycine, slightly lower to sarcosine and low activity to glycine Thalassiosira pseudonana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 sarcosine wild-type, pH 8.0, 25°C Thalassiosira pseudonana
0.85
-
 glycine wild-type, pH 8.0, 25°C Thalassiosira pseudonana
1.1
-
 N,N-dimethylglycine wild-type, pH 8.0, 25°C Thalassiosira pseudonana
1.2
-
 N,N-dimethylglycine truncation mutant carrying the C-terminal part, pH 8.0, 25°C Thalassiosira pseudonana
1.5
-
 sarcosine truncation mutant carrying the C-terminal part, pH 8.0, 25°C Thalassiosira pseudonana
2
-
 glycine truncation mutant carrying the N-terminal part, pH 8.0, 25°C Thalassiosira pseudonana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Thalassiosira pseudonana

Organism

Organism UniProt Comment Textmining
Thalassiosira pseudonana B8BQQ3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: alanine, methionine Thalassiosira pseudonana ?
-
-
S-adenosyl-L-methionine + glycine
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + sarcosine
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + betaine
-
-
S-adenosyl-L-methionine + sarcosine
-
 Thalassiosira pseudonana S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, calculated from sequence Thalassiosira pseudonana

Synonyms

Synonyms Comment Organism
THAPSDRAFT_20797
-
 Thalassiosira pseudonana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Thalassiosira pseudonana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate dimethylglycine Thalassiosira pseudonana
9
-
 substrates glycine, sarcosine Thalassiosira pseudonana

pI Value

Organism Comment pI Value Maximum pI Value
Thalassiosira pseudonana calculated from sequence
-
 4.9

Expression

Organism Comment Expression
Thalassiosira pseudonana expression is upregulated in high salinity up