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Literature summary for 2.1.1.160 extracted from

  • Mohanpuria, P.; Kumar, V.; Ahuja, P.S.; Yadav, S.K.
    Agrobacterium-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis L. (2011), Mol. Biotechnol., 48, 235-243.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS, method, overview Camellia sinensis

Protein Variants

Protein Variants Comment Organism
additional information Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS. The pFGC1008-CS contained 376 bp of caffeine synthase cDNA fragment in sense and antisense direction with an intron in between. Marked reduction in caffeine and theobromine contents in young shoots of tea seedlings occur after root transformation Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Camellia sinensis tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine ?
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis
-
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
?
S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
?

Organism

Organism UniProt Comment Textmining
Camellia sinensis B9VI89 cv. Kangra jat
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Camellia sinensis
-
root
-
Camellia sinensis
-
seedling
-
Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information tea caffeine synthase is a bifunctional enzyme comprising two S-adenosyl-L-methionine-dependent N-methyltransferase activities leading to the formation of theobromine and caffeine Camellia sinensis ?
-
?
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
?
S-adenosyl-L-methionine + 7-methylxanthine
-
Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
?

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Camellia sinensis