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Literature summary for 2.1.1.160 extracted from

  • Yoneyama, N.; Morimoto, H.; Ye, C.X.; Ashihara, H.; Mizuno, K.; Kato, M.
    Substrate specificity of N-methyltransferase involved in purine alkaloids synthesis is dependent upon one amino acid residue of the enzyme (2006), Mol. Genet. Genomics, 275, 125-135.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis Theobroma cacao
DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis Camellia ptilophylla
isozyme 1, DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis Camellia irrawadiensis

Protein Variants

Protein Variants Comment Organism
additional information construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview Theobroma cacao
additional information construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview Camellia irrawadiensis
additional information construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview Camellia ptilophylla

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 1,7-dimethylxanthine Theobroma cacao i.e. paraxanthine, alternative route in caffeine biosynthesis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine Camellia irrawadiensis i.e. paraxanthine, alternative route in caffeine biosynthesis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine Camellia ptilophylla i.e. paraxanthine, alternative route in caffeine biosynthesis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

Organism UniProt Comment Textmining
Camellia irrawadiensis Q2HXL9 isozyme 1
-
Camellia ptilophylla Q2HXI6
-
-
Theobroma cacao Q2HXL8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview Theobroma cacao ?
-
?
additional information substrate specificity, overview Camellia irrawadiensis ?
-
?
additional information substrate specificity, overview Camellia ptilophylla ?
-
?
S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine Camellia irrawadiensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine Camellia ptilophylla S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, alternative route in caffeine biosynthesis Theobroma cacao S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, alternative route in caffeine biosynthesis Camellia irrawadiensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, alternative route in caffeine biosynthesis Camellia ptilophylla S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
S-adenosyl-L-methionine + 1,7-dimethylxanthine i.e. paraxanthine, preferred substrate Theobroma cacao S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?