Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis | Theobroma cacao |
DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis | Camellia ptilophylla |
isozyme 1, DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis | Camellia irrawadiensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview | Theobroma cacao |
additional information | construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview | Camellia irrawadiensis |
additional information | construction and substrate specificity determination of chimeric mutant enzymes composed of theobromine synthase and inactive caffeine synthase, EC 2.1.1.160, overview | Camellia ptilophylla |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 1,7-dimethylxanthine | Theobroma cacao | i.e. paraxanthine, alternative route in caffeine biosynthesis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | Camellia irrawadiensis | i.e. paraxanthine, alternative route in caffeine biosynthesis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | Camellia ptilophylla | i.e. paraxanthine, alternative route in caffeine biosynthesis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia irrawadiensis | Q2HXL9 | isozyme 1 | - |
Camellia ptilophylla | Q2HXI6 | - |
- |
Theobroma cacao | Q2HXL8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview | Theobroma cacao | ? | - |
? | |
additional information | substrate specificity, overview | Camellia irrawadiensis | ? | - |
? | |
additional information | substrate specificity, overview | Camellia ptilophylla | ? | - |
? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine | Camellia irrawadiensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine | Camellia ptilophylla | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine, alternative route in caffeine biosynthesis | Theobroma cacao | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine, alternative route in caffeine biosynthesis | Camellia irrawadiensis | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine, alternative route in caffeine biosynthesis | Camellia ptilophylla | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? | |
S-adenosyl-L-methionine + 1,7-dimethylxanthine | i.e. paraxanthine, preferred substrate | Theobroma cacao | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ? |