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Literature summary for 2.1.1.159 extracted from

  • Waldhauser, S.S.M.; Gillies, F.M.; Crozier, A.; Baumann, T.W.
    Separation of the N-7 methyltransferase, the key enzyme in caffeine biosynthesis (1997), Phytochemistry, 45, 1407-1414.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Coffea arabica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
about, gel filtration Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?

Organism

Organism UniProt Comment Textmining
Coffea arabica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 39fold from young leaves by ammonium sulfate fractionation, 3 steps of ion exchange chromatography, and gel filtration Coffea arabica

Source Tissue

Source Tissue Comment Organism Textmining
leaf young Coffea arabica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.45
-
purified enzyme Coffea arabica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-methylxanthine
-
Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?

Subunits

Subunits Comment Organism
dimer 2 * 40000-49000, SDS-PAGE Coffea arabica

Synonyms

Synonyms Comment Organism
3NMT
-
Coffea arabica
N-3 methyltransferase
-
Coffea arabica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Coffea arabica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Coffea arabica

pI Value

Organism Comment pI Value Maximum pI Value
Coffea arabica chromatofocusing 5.1 4.5