Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Coffea arabica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
about, gel filtration | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 39fold from young leaves by ammonium sulfate fractionation, 3 steps of ion exchange chromatography, and gel filtration | Coffea arabica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Coffea arabica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.45 | - |
purified enzyme | Coffea arabica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? | |
S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000-49000, SDS-PAGE | Coffea arabica |
Synonyms | Comment | Organism |
---|---|---|
3NMT | - |
Coffea arabica |
N-3 methyltransferase | - |
Coffea arabica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Coffea arabica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Coffea arabica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Coffea arabica | chromatofocusing | 5.1 | 4.5 |