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Literature summary for 2.1.1.159 extracted from

  • Ogawa, M.; Herai, Y.; Koizumi, N.; Kusano, T.; Sano, H.
    7-Methylxanthine methyltransferase of coffee plants. Gene isolation and enzymatic properties (2001), J. Biol. Chem., 276, 8213-8218.
    View publication on PubMed
Search for more literature for 2.1.1.159

Cloned(Commentary)

Cloned (Comment) Organism
gene CaMTL2, cDNA library construction, DNA and amino acid sequence determination and analysis Coffea arabica
gene CaMXMT, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species, expression of CaMXMT as GST-fusion enzyme in Escherichia coli strain JM109 Coffea arabica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Coffea arabica 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway from xanthosine S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir

Organism

Organism UniProt Comment Textmining
Coffea arabica Q9AVJ9 gene CaMXMT, enzyme MXMT1; var. caturra
-
Coffea arabica Q9AVK1 gene CaMTL2; var. caturra
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography Coffea arabica

Source Tissue

Source Tissue Comment Organism Textmining
bud
-
 Coffea arabica
-
leaf high expression level of MXMT in young leaves, low expression level in old leaves Coffea arabica
-
leaf old and young Coffea arabica
-
additional information wide tissue distribution Coffea arabica
-
root
-
 Coffea arabica
-
root low expression level of MXMT Coffea arabica
-
stem
-
 Coffea arabica
-
stem high expression level of MXMT Coffea arabica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-methylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway from xanthosine Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ir
S-adenosyl-L-methionine + paraxanthine low activity Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ir

Synonyms

Synonyms Comment Organism
7-methylxanthine methyltransferase
-
 Coffea arabica
7-methylxanthine N-methyltransferase
-
 Coffea arabica
MXMT
-
 Coffea arabica
MXMT1
-
 Coffea arabica
N-methyltransferase
-
 Coffea arabica