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Literature summary for 2.1.1.158 extracted from
- Essential region for 3-N methylation in N-methyltransferases involved in caffeine biosynthesis (2010), Z. Naturforsch. C, 65c, 257-265.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparison of caffeine synthetic enzymes from coffee | Coffea sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A23S | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| A23S/L191P/H219R | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| A23S/P104Q/H219R | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| A23S/P104Q/Q161H/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows 60% of wild-type 7-methylation activity | Coffea sp. |
| A23S/P104Q/Q161H/L191P/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| A23S/Q161H/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| A23S/Q161H/L191P/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| H219R | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| L191P | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| additional information | construction of several enzyme mutants, overview. The mutants of CmXRS1, that have 3-N methylation activity and produce caffeine from paraxanthine as a substrate, need to have replacement of the glutamine residue by histidine at position 161 in the CmXRS1 sequence, i.e. a Q161H mutation, overview | Coffea sp. |
| P104Q | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| P104Q/L191P | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| P104Q/Q161H | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| P104Q/Q161H/L191P | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| Q161H | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
| Q161H/L191P | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Coffea sp. |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + xanthosine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coffea sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | 7-methylxanthosine synthase shows the most critical substrate specifi city of the three types of N-methyltransferases in Coffea species | Coffea sp. | ? | - |
? | |
| S-adenosyl-L-methionine + xanthosine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CmXRS1 | - |
Coffea sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Coffea sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Coffea sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Coffea sp. | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme catalyzes the first step in caffeine biosynthesis, pathway overview | Coffea sp. |
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Release 2023.1 (January 2023)
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