BRENDA - Enzyme Database show
show all sequences of 2.1.1.158

Separation of the N-7 methyltransferase, the key enzyme in caffeine biosynthesis

Waldhauser, S.S.M.; Gillies, F.M.; Crozier, A.; Baumann, T.W.; Phytochemistry 45, 1407-1414 (1997)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Coffea arabica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Coffea arabica
67000
-
about, gel filtration
Coffea arabica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + xanthosine
Coffea arabica
first step in caffeine biosynthesis pathway from xanthosine
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coffea arabica
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme 9fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration
Coffea arabica
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
young
Coffea arabica
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.32
-
purified enzyme
Coffea arabica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + xanthosine
-
670509
Coffea arabica
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
?
S-adenosyl-L-methionine + xanthosine
first step in caffeine biosynthesis pathway from xanthosine
670509
Coffea arabica
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Coffea arabica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Coffea arabica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Coffea arabica
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Coffea arabica
chromatofocusing
5.2
4.7
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Coffea arabica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Coffea arabica
67000
-
about, gel filtration
Coffea arabica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + xanthosine
Coffea arabica
first step in caffeine biosynthesis pathway from xanthosine
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 9fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration
Coffea arabica
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
young
Coffea arabica
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.32
-
purified enzyme
Coffea arabica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + xanthosine
-
670509
Coffea arabica
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
?
S-adenosyl-L-methionine + xanthosine
first step in caffeine biosynthesis pathway from xanthosine
670509
Coffea arabica
S-adenosyl-L-homocysteine + 7-methylxanthosine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Coffea arabica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Coffea arabica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Coffea arabica
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Coffea arabica
chromatofocusing
5.2
4.7
Other publictions for EC 2.1.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721124
Mizuno
Essential region for 3-N methy ...
Coffea sp.
Z. Naturforsch. C
65c
257-265
2010
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1
-
16
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1
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1
-
2
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2
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1
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-
1
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-
1
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1
1
-
16
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1
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1
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-
-
-
2
-
1
-
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-
1
-
-
-
-
1
1
-
-
-
696486
Kodama
Dimerization of N-methyltransf ...
Coffea arabica
Biochimie
90
547-551
2008
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-
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1
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1
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1
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1
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700656
Deng
-
Fine control of caffeine biosy ...
Camellia sinensis
Phytochem. Lett.
1
195-198
2008
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-
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1
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1
1
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667101
McCarthy
Cloning, expression, crystalli ...
Coffea canephora
Acta Crystallogr. Sect. F
F63
304-307
2007
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1
1
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2
1
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1
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1
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1
1
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1
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1
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1
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1
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1
1
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670598
McCarthy
The structure of two N-methylt ...
Coffea canephora
Plant Physiol.
144
879-889
2007
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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684218
McCarthy
Cloning, expression, crystalli ...
Coffea canephora
Acta Crystallogr. Sect. F
63
304-307
2007
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-
1
1
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2
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1
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1
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1
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1
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1
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2
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1
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1
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670621
Koshiro
-
Changes in content and biosynt ...
Coffea arabica, Coffea canephora
Plant Sci.
171
242-250
2006
-
-
-
-
-
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-
-
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6
-
2
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8
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9
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6
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8
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9
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670568
Uefuji
Caffeine production in tobacco ...
Coffea arabica
Plant Mol. Biol.
59
221-227
2005
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1
1
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1
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1
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3
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1
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1
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670565
Ogita
Application of RNAi to confirm ...
Coffea arabica, Coffea canephora
Plant Mol. Biol.
54
931-941
2004
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2
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2
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2
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6
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6
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4
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2
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2
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2
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6
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4
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668586
Mizuno
The first committed step react ...
Coffea arabica
FEBS Lett.
547
56-60
2003
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1
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1
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1
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4
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4
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3
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1
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1
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1
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4
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3
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670578
Uefuji
Molecular cloning and function ...
Coffea arabica
Plant Physiol.
132
372-380
2003
-
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1
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1
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2
1
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1
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3
1
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1
1
1
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1
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1
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1
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2
1
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3
1
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1
1
1
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1
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655545
Koshiishi
A new caffeine biosynthetic pa ...
Camellia sinensis
FEBS Lett.
499
50-54
2001
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1
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2
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1
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1
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2
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1
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1
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669194
Ogawa
7-Methylxanthine methyltransfe ...
Coffea arabica
J. Biol. Chem.
276
8213-8218
2001
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1
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1
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1
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3
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1
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3
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667105
Moisyadi
-
Cloning and characterization o ...
Coffea arabica
Acta Hortic.
461
367-377
1998
-
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1
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1
1
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1
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1
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1
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1
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1
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1
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2
1
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1
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670509
Waldhauser
Separation of the N-7 methyltr ...
Coffea arabica
Phytochemistry
45
1407-1414
1997
-
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-
-
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1
2
1
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2
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1
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1
1
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2
1
1
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1
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1
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1
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1
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1
1
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1
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1
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667116
Negishi
-
The role of xanthosine in the ...
Coffea arabica
Agric. Biol. Chem.
49
2221-2222
1985
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