BRENDA - Enzyme Database show
show all sequences of 2.1.1.156

Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus

Kimura, Y.; Kawasaki, S.; Yoshimoto, H.; Takegawa, K.; J. Bacteriol. 192, 1467-1470 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene gsmA, DNA and amino acid sequence determination and analysis, sequence comparison
Myxococcus xanthus
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of a chromosomal gsmA gene disruption knockout mutant, phenotype, overview
Myxococcus xanthus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29500
-
x * 29500, about, sequence calculation
Myxococcus xanthus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + glycine
Myxococcus xanthus
-
S-adenosyl-L-homocysteine + sarcosine
-
-
?
S-adenosyl-L-methionine + sarcosine
Myxococcus xanthus
-
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Myxococcus xanthus
-
gene gsmA encodes a Gsm homologue protein
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + glycine
-
719715
Myxococcus xanthus
S-adenosyl-L-homocysteine + sarcosine
-
-
-
?
S-adenosyl-L-methionine + sarcosine
-
719715
Myxococcus xanthus
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 29500, about, sequence calculation
Myxococcus xanthus
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Myxococcus xanthus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene gsmA, DNA and amino acid sequence determination and analysis, sequence comparison
Myxococcus xanthus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Myxococcus xanthus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of a chromosomal gsmA gene disruption knockout mutant, phenotype, overview
Myxococcus xanthus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29500
-
x * 29500, about, sequence calculation
Myxococcus xanthus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + glycine
Myxococcus xanthus
-
S-adenosyl-L-homocysteine + sarcosine
-
-
?
S-adenosyl-L-methionine + sarcosine
Myxococcus xanthus
-
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + glycine
-
719715
Myxococcus xanthus
S-adenosyl-L-homocysteine + sarcosine
-
-
-
?
S-adenosyl-L-methionine + sarcosine
-
719715
Myxococcus xanthus
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29500, about, sequence calculation
Myxococcus xanthus
General Information
General Information
Commentary
Organism
malfunction
disruption of the gsmA gene, encoding Gsm homologue protein, generates a mutant that exhibits a longer lag period of growth and delayed spore germination under osmostress, it shows normal growth in CYE medium at 30°C, phenotype, overview
Myxococcus xanthus
metabolism
glycine sarcosine methyltransferase and sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, catalyze glycine betaine synthesis from glycine
Myxococcus xanthus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
disruption of the gsmA gene, encoding Gsm homologue protein, generates a mutant that exhibits a longer lag period of growth and delayed spore germination under osmostress, it shows normal growth in CYE medium at 30°C, phenotype, overview
Myxococcus xanthus
metabolism
glycine sarcosine methyltransferase and sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, catalyze glycine betaine synthesis from glycine
Myxococcus xanthus
Other publictions for EC 2.1.1.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733736
Niu
-
Co-expression of ApGSMT and Ap ...
Aphanothece halophytica
Environ. Exp. Bot.
104
16-25
2014
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1
-
-
-
-
-
-
-
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2
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1
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2
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1
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2
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2
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1
1
-
-
-
734932
Lai
Transgenic Arabidopsis express ...
Methanohalophilus portucalensis, Methanohalophilus portucalensis FDF-1
Plant Mol. Biol.
85
429-441
2014
-
-
1
-
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-
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-
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4
-
5
-
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4
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1
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4
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4
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1
1
-
-
-
734671
He
Co-expression of genes ApGSMT2 ...
Aphanothece halophytica
Mol. Breed.
31
559-573
2013
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-
1
-
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2
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1
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2
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1
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2
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2
-
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-
1
1
1
1
-
-
720836
Lai
Characterization and regulatio ...
Methanohalophilus portucalensis, Methanohalophilus portucalensis FDF1
PLoS ONE
6
e25090
2011
-
-
1
-
-
-
2
4
-
3
2
6
-
2
-
-
1
-
-
-
-
-
8
1
1
-
-
-
1
-
-
1
-
1
2
-
-
1
1
-
-
-
2
2
-
4
-
3
2
6
-
-
-
1
-
-
-
-
8
1
1
-
-
-
1
-
-
1
1
2
2
1
-
-
719715
Kimura
Glycine betaine biosynthesized ...
Myxococcus xanthus
J. Bacteriol.
192
1467-1470
2010
-
-
1
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
1
-
-
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-
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-
-
1
-
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1
1
-
1
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-
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1
2
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2
1
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-
-
-
-
-
-
2
2
-
-
-
671647
Lu
Identification of glycine beta ...
Synechococcus sp. WH 8102
Arch. Microbiol.
186
495-506
2006
-
-
1
-
-
-
-
4
-
-
3
2
-
6
-
-
1
-
-
-
-
-
5
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
3
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
659286
Waditee
Isolation and functional chara ...
Aphanothece halophytica
J. Biol. Chem.
278
4932-4942
2003
1
-
1
-
2
-
12
9
-
-
3
5
-
2
-
-
1
-
-
-
-
-
9
2
1
-
-
-
2
1
-
-
-
-
-
1
-
2
-
-
4
-
-
18
-
12
-
-
5
5
-
-
-
2
-
-
-
-
9
3
1
-
-
-
2
1
-
-
-
-
-
-
-
-
657578
Nyyssola
Characterization of glycine sa ...
Halorhodospira halochloris
Appl. Environ. Microbiol.
67
2044-2050
2001
1
3
1
-
-
-
5
4
-
1
3
3
-
3
-
-
1
-
-
-
3
-
6
1
1
-
-
-
2
-
-
-
-
-
-
1
3
1
-
-
-
-
-
5
-
4
-
1
3
3
-
-
-
1
-
-
3
-
6
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
659145
Nyyssola
Extreme halophiles synthesize ...
Actinopolyspora halophila, Halorhodospira halochloris
J. Biol. Chem.
275
22196-22201
2000
-
-
2
-
-
1
-
-
-
-
1
6
-
3
-
-
1
-
-
-
6
-
13
1
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
6
-
-
-
1
-
-
6
-
13
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-