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Literature summary for 2.1.1.156 extracted from

  • Lu, W.D.; Chi, Z.M.; Su, C.D.
    Identification of glycine betaine as compatible solute in Synechococcus sp. WH8102 and characterization of its N-methyltransferase genes involved in betaine synthesis (2006), Arch. Microbiol., 186, 495-506.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS Parasynechococcus marenigrum WH 8102

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
S-adenosyl-L-methionine with glycine as methyl group acceptor, pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
0.44
-
S-adenosyl-L-methionine with N-methylglycine as methyl group acceptor, pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
2.94
-
N-methylglycine pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
5.22
-
glycine pH 8.2, 37°C Parasynechococcus marenigrum WH 8102

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration Parasynechococcus marenigrum WH 8102
35500
-
gel filtration Parasynechococcus marenigrum WH 8102
38000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration Parasynechococcus marenigrum WH 8102

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + glycine Parasynechococcus marenigrum WH 8102 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + N-methylglycine Parasynechococcus marenigrum WH 8102 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?

Organism

Organism UniProt Comment Textmining
Parasynechococcus marenigrum WH 8102 Q7U4Z8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Parasynechococcus marenigrum WH 8102

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information several other amino acids tested as substrates and non of them serves as methyl group acceptor Parasynechococcus marenigrum WH 8102 ?
-
?
S-adenosyl-L-methionine + glycine
-
Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + glycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + N-methylglycine
-
Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
S-adenosyl-L-methionine + N-methylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration Parasynechococcus marenigrum WH 8102

Synonyms

Synonyms Comment Organism
glycine sarcosine N-methyltransferase
-
Parasynechococcus marenigrum WH 8102

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
with N-methylglycine as substrate Parasynechococcus marenigrum WH 8102
8.2
-
with glycine as substrate Parasynechococcus marenigrum WH 8102

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Parasynechococcus marenigrum WH 8102