BRENDA - Enzyme Database show
show all sequences of 2.1.1.156

Identification of glycine betaine as compatible solute in Synechococcus sp. WH8102 and characterization of its N-methyltransferase genes involved in betaine synthesis

Lu, W.D.; Chi, Z.M.; Su, C.D.; Arch. Microbiol. 186, 495-506 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
Synechococcus sp. WH 8102
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
S-adenosyl-L-methionine
with glycine as methyl group acceptor, pH 8.2, 37C
Synechococcus sp. WH 8102
0.44
-
S-adenosyl-L-methionine
with N-methylglycine as methyl group acceptor, pH 8.2, 37C
Synechococcus sp. WH 8102
2.94
-
N-methylglycine
pH 8.2, 37C
Synechococcus sp. WH 8102
5.22
-
glycine
pH 8.2, 37C
Synechococcus sp. WH 8102
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration
Synechococcus sp. WH 8102
35500
-
gel filtration
Synechococcus sp. WH 8102
38000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration
Synechococcus sp. WH 8102
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + glycine
Synechococcus sp. WH 8102
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
S-adenosyl-L-homocysteine + N-methylglycine
-
-
?
S-adenosyl-L-methionine + N-methylglycine
Synechococcus sp. WH 8102
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechococcus sp. WH 8102
Q7U4Z8
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein using His-tag
Synechococcus sp. WH 8102
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
several other amino acids tested as substrates and non of them serves as methyl group acceptor
671647
Synechococcus sp. WH 8102
?
-
-
-
-
S-adenosyl-L-methionine + glycine
-
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N-methylglycine
-
-
-
?
S-adenosyl-L-methionine + glycine
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N-methylglycine
-
-
-
?
S-adenosyl-L-methionine + N-methylglycine
-
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
-
?
S-adenosyl-L-methionine + N-methylglycine
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration
Synechococcus sp. WH 8102
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
with N-methylglycine as substrate
Synechococcus sp. WH 8102
8.2
-
with glycine as substrate
Synechococcus sp. WH 8102
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Synechococcus sp. WH 8102
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
Synechococcus sp. WH 8102
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
S-adenosyl-L-methionine
with glycine as methyl group acceptor, pH 8.2, 37C
Synechococcus sp. WH 8102
0.44
-
S-adenosyl-L-methionine
with N-methylglycine as methyl group acceptor, pH 8.2, 37C
Synechococcus sp. WH 8102
2.94
-
N-methylglycine
pH 8.2, 37C
Synechococcus sp. WH 8102
5.22
-
glycine
pH 8.2, 37C
Synechococcus sp. WH 8102
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration
Synechococcus sp. WH 8102
35500
-
gel filtration
Synechococcus sp. WH 8102
38000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration
Synechococcus sp. WH 8102
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + glycine
Synechococcus sp. WH 8102
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
S-adenosyl-L-homocysteine + N-methylglycine
-
-
?
S-adenosyl-L-methionine + N-methylglycine
Synechococcus sp. WH 8102
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein using His-tag
Synechococcus sp. WH 8102
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
several other amino acids tested as substrates and non of them serves as methyl group acceptor
671647
Synechococcus sp. WH 8102
?
-
-
-
-
S-adenosyl-L-methionine + glycine
-
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N-methylglycine
-
-
-
?
S-adenosyl-L-methionine + glycine
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N-methylglycine
-
-
-
?
S-adenosyl-L-methionine + N-methylglycine
-
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
-
?
S-adenosyl-L-methionine + N-methylglycine
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
671647
Synechococcus sp. WH 8102
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration
Synechococcus sp. WH 8102
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
with N-methylglycine as substrate
Synechococcus sp. WH 8102
8.2
-
with glycine as substrate
Synechococcus sp. WH 8102
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Synechococcus sp. WH 8102
Other publictions for EC 2.1.1.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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2
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2
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4
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4
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1
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4
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4
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734671
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2
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1
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2
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2
-
-
-
-
-
-
-
-
-
1
1
1
1
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-
720836
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PLoS ONE
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1
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2
4
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3
2
6
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2
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1
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-
-
-
8
1
1
-
-
-
1
-
-
1
-
1
2
-
-
1
1
-
-
-
2
2
-
4
-
3
2
6
-
-
-
1
-
-
-
-
8
1
1
-
-
-
1
-
-
1
1
2
2
1
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-
719715
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Glycine betaine biosynthesized ...
Myxococcus xanthus
J. Bacteriol.
192
1467-1470
2010
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1
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1
-
-
-
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-
1
2
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1
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-
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-
-
-
-
-
2
1
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-
1
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-
1
1
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1
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1
2
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-
2
1
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-
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2
2
-
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-
671647
Lu
Identification of glycine beta ...
Synechococcus sp. WH 8102
Arch. Microbiol.
186
495-506
2006
-
-
1
-
-
-
-
4
-
-
3
2
-
6
-
-
1
-
-
-
-
-
5
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
3
2
-
-
-
1
-
-
-
-
5
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
659286
Waditee
Isolation and functional chara ...
Aphanothece halophytica
J. Biol. Chem.
278
4932-4942
2003
1
-
1
-
2
-
12
9
-
-
3
5
-
2
-
-
1
-
-
-
-
-
9
2
1
-
-
-
2
1
-
-
-
-
-
1
-
2
-
-
4
-
-
18
-
12
-
-
5
5
-
-
-
2
-
-
-
-
9
3
1
-
-
-
2
1
-
-
-
-
-
-
-
-
657578
Nyyssola
Characterization of glycine sa ...
Halorhodospira halochloris
Appl. Environ. Microbiol.
67
2044-2050
2001
1
3
1
-
-
-
5
4
-
1
3
3
-
3
-
-
1
-
-
-
3
-
6
1
1
-
-
-
2
-
-
-
-
-
-
1
3
1
-
-
-
-
-
5
-
4
-
1
3
3
-
-
-
1
-
-
3
-
6
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
659145
Nyyssola
Extreme halophiles synthesize ...
Actinopolyspora halophila, Halorhodospira halochloris
J. Biol. Chem.
275
22196-22201
2000
-
-
2
-
-
1
-
-
-
-
1
6
-
3
-
-
1
-
-
-
6
-
13
1
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
6
-
-
-
1
-
-
6
-
13
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-