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Literature summary for 2.1.1.156 extracted from

  • Nyyssola, A.; Kerovuo, J.; Kaukinen, P.; von Weymarn, N.; Reinikainen, T.
    Extreme halophiles synthesize betaine from glycine by methylation (2000), J. Biol. Chem., 275, 22196-22201.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, functional coexpression in Escherichia coli with sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, conferring betaine accumulation and increased salt tolerance to the Escherichia coli cells Halorhodospira halochloris
DNA and amino acid sequence determination, the single gene encodes the glycine sarcosine methyltransferase as well as the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, the latter being probably a proteolytic processing product of the first, poor expression in Escherichia coli of the full length and the truncated enzyme Actinopolyspora halophila

General Stability

General Stability Organism
enzyme is instable during purification, activity is lost within 30 min Actinopolyspora halophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Halorhodospira halochloris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Halorhodospira halochloris the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process ?
-
?
additional information Actinopolyspora halophila the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process ?
-
?
S-adenosyl-L-methionine + glycine Halorhodospira halochloris
-
S-adenosyl-L-homocysteine + sarcosine
-
?
S-adenosyl-L-methionine + glycine Actinopolyspora halophila
-
S-adenosyl-L-homocysteine + sarcosine
-
?
S-adenosyl-L-methionine + sarcosine Halorhodospira halochloris
-
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
S-adenosyl-L-methionine + sarcosine Actinopolyspora halophila
-
S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?

Organism

Organism UniProt Comment Textmining
Actinopolyspora halophila Q9KJ20
-
-
Halorhodospira halochloris
-
-
-
Halorhodospira halochloris Q9KJ22 i.e. Halorhodospira halochloris, ATCC 35916
-

Purification (Commentary)

Purification (Comment) Organism
by hydrophobic interaction chromatography, ammonium sulfate fractionation, gel filtration, ion exchange chromatography and ultrafiltration Halorhodospira halochloris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.023
-
activity with glycine and S-adenosyl-L-methionine in crude cell extracts Actinopolyspora halophila
0.058
-
activity with glycine and S-adenosyl-L-methionine in crude cell extracts Halorhodospira halochloris
0.19
-
activity with sarcosine and S-adenosyl-L-methionine, purified enzyme Halorhodospira halochloris
0.23
-
activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts Halorhodospira halochloris
0.34
-
activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts Actinopolyspora halophila
0.52
-
activity with glycine and S-adenosyl-L-methionine, purified enzyme Halorhodospira halochloris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + glycine overall reaction Halorhodospira halochloris 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
additional information the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process Halorhodospira halochloris ?
-
?
additional information the enzyme catalyzes the first 2 steps of betaine synthesis from glycine in a 3-step process Actinopolyspora halophila ?
-
?
additional information the enzyme has overlapping activities with the enzyme catalyzing the following reaction steps Halorhodospira halochloris ?
-
?
additional information the enzyme has overlapping activities with the enzyme catalyzing the following reaction steps Actinopolyspora halophila ?
-
?
S-adenosyl-L-methionine + glycine
-
Halorhodospira halochloris S-adenosyl-L-homocysteine + sarcosine
-
?
S-adenosyl-L-methionine + glycine
-
Actinopolyspora halophila S-adenosyl-L-homocysteine + sarcosine
-
?
S-adenosyl-L-methionine + glycine
-
Halorhodospira halochloris S-adenosyl-L-homocysteine + sarcosine i.e. 2-methylglycine ?
S-adenosyl-L-methionine + sarcosine
-
Halorhodospira halochloris S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
S-adenosyl-L-methionine + sarcosine
-
Actinopolyspora halophila S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
S-adenosyl-L-methionine + sarcosine i.e. 2-methylglycine Actinopolyspora halophila S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Halorhodospira halochloris

Synonyms

Synonyms Comment Organism
glycine sarcosine methyltransferase
-
Halorhodospira halochloris
glycine sarcosine methyltransferase
-
Actinopolyspora halophila
GSMT
-
Halorhodospira halochloris
GSMT
-
Actinopolyspora halophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Halorhodospira halochloris
37
-
assay at Actinopolyspora halophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Halorhodospira halochloris
7.5
-
assay at Actinopolyspora halophila