BRENDA - Enzyme Database show
show all sequences of 2.1.1.150

Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase

He, X.Z.; Reddy, J.T.; Dixon, R.A.; Plant Mol. Biol. 36, 43-54 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Medicago sativa
Engineering
Amino acid exchange
Commentary
Organism
up
IOMT transcripts are rapidly induced following elicitation, prior to the increase in IOMT activity
Medicago sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39603
-
x * 39603, calculated from sequence
Medicago sativa
41000
-
x * 41000, SDS-PAGE
Medicago sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
Medicago sativa
i.e. daidzein
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. isoformononetin
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago sativa
O24529
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
callus
-
Medicago sativa
-
cell suspension culture
-
Medicago sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
i.e. genistein. 12.9% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
441447
Medicago sativa
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
136% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
441447
Medicago sativa
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
441447
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. isoformononetin
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 39603, calculated from sequence; x * 41000, SDS-PAGE
Medicago sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Medicago sativa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
up
IOMT transcripts are rapidly induced following elicitation, prior to the increase in IOMT activity
Medicago sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39603
-
x * 39603, calculated from sequence
Medicago sativa
41000
-
x * 41000, SDS-PAGE
Medicago sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
Medicago sativa
i.e. daidzein
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. isoformononetin
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
callus
-
Medicago sativa
-
cell suspension culture
-
Medicago sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
i.e. genistein. 12.9% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
441447
Medicago sativa
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
136% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
441447
Medicago sativa
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
441447
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. isoformononetin
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 39603, calculated from sequence; x * 41000, SDS-PAGE
Medicago sativa
Other publictions for EC 2.1.1.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676552
Deavours
Functional analysis of members ...
Medicago truncatula
Plant Mol. Biol.
62
715-733
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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16
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16
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
441448
Liu
Elicitor-induced association o ...
Medicago sativa, Medicago sativa cv Regen SY
Plant Cell
13
2643-2658
2001
-
-
-
-
-
-
-
-
1
-
-
2
-
4
-
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-
1
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4
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1
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1
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1
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2
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1
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4
-
1
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-
1
-
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-
643765
Zubieta
Structures of two natural prod ...
Medicago sativa
Nat. Struct. Biol.
8
271-279
2001
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-
1
1
-
-
-
-
-
-
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3
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-
1
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-
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-
1
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1
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1
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1
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1
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441445
He
Genetic manipulation of isofla ...
Medicago sativa, Medicago sativa cv Regen SY
Plant Cell
12
1689-1702
2000
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-
-
-
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2
-
4
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4
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2
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4
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-
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-
441447
He
Stress responses in alfalfa (M ...
Medicago sativa
Plant Mol. Biol.
36
43-54
1998
-
-
1
-
1
-
-
-
-
-
2
1
-
6
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-
2
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3
1
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1
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1
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2
1
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2
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3
1
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441446
He
Affinity chromatography, subst ...
Medicago sativa
Arch. Biochem. Biophys.
336
121-129
1996
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1
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4
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1
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2
1
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5
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1
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1
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1
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1
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2
1
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5
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1
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1
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-
389479
Dalkin
Stress responses in alfalfa (M ...
Medicago sativa
Plant Physiol.
92
440-446
1990
2
-
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-
1
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1
1
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4
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1
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-
1
-
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5
1
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-
-
2
-
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-
2
-
2
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-
-
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-
1
-
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-
1
1
-
-
-
-
1
-
1
-
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5
1
-
-
-
-
2
-
-
2
1
-
-
1
-
-