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Literature summary for 2.1.1.150 extracted from

  • He, X.Z.; Dixon, R.A.
    Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.) (1996), Arch. Biochem. Biophys., 336, 121-129.
    View publication on PubMed
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein). Key enzyme in the biosynthesis of the phytoalexin medicarpin in Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Organism

Organism UniProt Comment Textmining
Medicago sativa O24529
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
 Medicago sativa
-
cell suspension culture
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
 pH 8.5, 30°C Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with apigenin (a flavone), naringenine (a falvanone) Medicago sativa ?
-
 ?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein, about 7% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein) Medicago sativa S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone (i.e. glycitein) Medicago sativa S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein). Key enzyme in the biosynthesis of the phytoalexin medicarpin in Medicago sativa Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein, about 20% of the activity with 6,7,4'-trihydroxyisoflavone (i.e. glycitein) Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Synonyms

Synonyms Comment Organism
8-IOMT
-
 Medicago sativa
isoflavone O-methyltransferase
-
 Medicago sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 glycine-NaOH buffer Medicago sativa