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Literature summary for 2.1.1.150 extracted from

  • He, X.Z.; Dixon, R.A.
    Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa (2000), Plant Cell, 12, 1689-1702.
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Medicago sativa cv Regen SY i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-
Medicago sativa cv Regen SY
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein Medicago sativa cv Regen SY S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected Medicago sativa cv Regen SY S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?