BRENDA - Enzyme Database show
show all sequences of 2.1.1.150

Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa

He, X.Z.; Dixon, R.A.; Plant Cell 12, 1689-1702 (2000)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
Medicago sativa
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
Medicago sativa cv Regen SY
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago sativa
-
-
-
Medicago sativa cv Regen SY
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
441445
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
441445
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
441445
Medicago sativa cv Regen SY
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
441445
Medicago sativa cv Regen SY
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
Medicago sativa
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
Medicago sativa cv Regen SY
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
441445
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
441445
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
441445
Medicago sativa cv Regen SY
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein. 4'-O-Methylation of an isoflavonoid intermediate is a key reaction in the biosynthesis of the phytoalexin medicarpin in legumes. However, isoflavone O-methyltransferase from alfalfa converts the isoflavone daidzein to 7-O-methyldaidzein (isoformononetin) in vitro as well as in vivo in unchallenged leaves of transgenic alfalfa ectopically expressing IOMT. In contrast, elicitation of IOMT-overexpressing plants with CuC2 or infecting these plants with Phoma medicaginis leads to greater accumulation of formononetin (4'-O-methyl daidzein) and medicarpin in the leaves than does elicitation or infection of control plants, and no isoformononetin is detected
441445
Medicago sativa cv Regen SY
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. formononetin
-
-
?
Other publictions for EC 2.1.1.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676552
Deavours
Functional analysis of members ...
Medicago truncatula
Plant Mol. Biol.
62
715-733
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441448
Liu
Elicitor-induced association o ...
Medicago sativa, Medicago sativa cv Regen SY
Plant Cell
13
2643-2658
2001
-
-
-
-
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
643765
Zubieta
Structures of two natural prod ...
Medicago sativa
Nat. Struct. Biol.
8
271-279
2001
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441445
He
Genetic manipulation of isofla ...
Medicago sativa, Medicago sativa cv Regen SY
Plant Cell
12
1689-1702
2000
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441447
He
Stress responses in alfalfa (M ...
Medicago sativa
Plant Mol. Biol.
36
43-54
1998
-
-
1
-
1
-
-
-
-
-
2
1
-
6
-
-
-
-
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441446
He
Affinity chromatography, subst ...
Medicago sativa
Arch. Biochem. Biophys.
336
121-129
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
2
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
2
1
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
389479
Dalkin
Stress responses in alfalfa (M ...
Medicago sativa
Plant Physiol.
92
440-446
1990
2
-
-
-
-
1
-
-
-
1
1
-
-
4
-
-
1
-
-
1
-
-
5
1
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
1
-
-
5
1
-
-
-
-
2
-
-
2
1
-
-
1
-
-