BRENDA - Enzyme Database show
show all sequences of 2.1.1.150

Stress responses in alfalfa (Medicago sativa L.). I. Induction of phenylpropanoid biosynthesis and hydrolytic enzymes in elicitor-treated cell suspension cultures

Dalkin, K.; Edwards, R.; Edington, B.; Dixon, R.A.; Plant Physiol. 92, 440-446 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithioerythritol
10-20 mM, up to 39% inhibition
Medicago sativa
dithiothreitol
10-20 mM, up to 39% inhibition
Medicago sativa
General Stability
General Stability
Organism
stabilized in the presence of EDTA and thiol-containing reagents
Medicago sativa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
enzyme activity was not affected by inclusion of 5 mM Mn2+, Mg2+, or EDTA in the assay mixture
Medicago sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
1 * 41000, SDS-PAGE
Medicago sativa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago sativa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Medicago sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Medicago sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
IOMT II does not catalyse the methylation of apigenin, kaempferol, flavonoids mono-hydroxylated in the 2’,3,4’,5, or 7 positions, flavanones such as naringenin or coumestans such as coumestrol
389479
Medicago sativa
?
-
-
-
-
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
i.e. genistein. 50% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
389479
Medicago sativa
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
i.e. glycitein. 178% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
389479
Medicago sativa
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,2'-dihydroxy-4'-methoxyisoflavone
i.e. 2'-hydroxyformononetin. 55% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
389479
Medicago sativa
S-adenosyl-L-homocysteine + 2-hydroxy-7,4'-dimethoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
389479
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. isoformononetin
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 41000, SDS-PAGE
Medicago sativa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
Tris buffer
Medicago sativa
8.7
-
glycine-NaOH buffer
Medicago sativa
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Medicago sativa
IOMT IIB, chromatography
-
4.3
Medicago sativa
IOMT IIA, chromatography
-
4.9
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithioerythritol
10-20 mM, up to 39% inhibition
Medicago sativa
dithiothreitol
10-20 mM, up to 39% inhibition
Medicago sativa
General Stability (protein specific)
General Stability
Organism
stabilized in the presence of EDTA and thiol-containing reagents
Medicago sativa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
enzyme activity was not affected by inclusion of 5 mM Mn2+, Mg2+, or EDTA in the assay mixture
Medicago sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
1 * 41000, SDS-PAGE
Medicago sativa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Medicago sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Medicago sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
IOMT II does not catalyse the methylation of apigenin, kaempferol, flavonoids mono-hydroxylated in the 2’,3,4’,5, or 7 positions, flavanones such as naringenin or coumestans such as coumestrol
389479
Medicago sativa
?
-
-
-
-
S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone
i.e. genistein. 50% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
389479
Medicago sativa
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone
i.e. glycitein. 178% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
389479
Medicago sativa
S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,2'-dihydroxy-4'-methoxyisoflavone
i.e. 2'-hydroxyformononetin. 55% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein)
389479
Medicago sativa
S-adenosyl-L-homocysteine + 2-hydroxy-7,4'-dimethoxyisoflavone
-
-
-
?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone
i.e. daidzein
389479
Medicago sativa
S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
i.e. isoformononetin
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 41000, SDS-PAGE
Medicago sativa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
Tris buffer
Medicago sativa
8.7
-
glycine-NaOH buffer
Medicago sativa
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Medicago sativa
IOMT IIB, chromatography
-
4.3
Medicago sativa
IOMT IIA, chromatography
-
4.9
Expression
Organism
Commentary
Expression
Medicago sativa
treatment of alfalfa cell suspension cultures with elicitor preparations from Saccharomyces cerevisiae or from cell walls of Colletotrichum lindemuthianum results in a ca 200fold induction of isoflavone 0-methyltransferase activity
up
Expression (protein specific)
Organism
Commentary
Expression
Medicago sativa
treatment of alfalfa cell suspension cultures with elicitor preparations from Saccharomyces cerevisiae or from cell walls of Colletotrichum lindemuthianum results in a ca 200fold induction of isoflavone 0-methyltransferase activity
up
Other publictions for EC 2.1.1.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676552
Deavours
Functional analysis of members ...
Medicago truncatula
Plant Mol. Biol.
62
715-733
2006
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-
-
-
-
-
-
-
-
-
-
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3
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16
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16
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-
441448
Liu
Elicitor-induced association o ...
Medicago sativa, Medicago sativa cv Regen SY
Plant Cell
13
2643-2658
2001
-
-
-
-
-
-
-
-
1
-
-
2
-
4
-
-
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-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
1
-
-
2
-
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-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
643765
Zubieta
Structures of two natural prod ...
Medicago sativa
Nat. Struct. Biol.
8
271-279
2001
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-
1
1
-
-
-
-
-
-
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3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
-
-
-
-
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-
-
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-
1
-
-
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-
1
-
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-
-
-
441445
He
Genetic manipulation of isofla ...
Medicago sativa, Medicago sativa cv Regen SY
Plant Cell
12
1689-1702
2000
-
-
-
-
-
-
-
-
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-
-
2
-
4
-
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-
4
-
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-
-
-
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2
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4
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-
-
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-
441447
He
Stress responses in alfalfa (M ...
Medicago sativa
Plant Mol. Biol.
36
43-54
1998
-
-
1
-
1
-
-
-
-
-
2
1
-
6
-
-
-
-
-
2
-
-
3
1
-
-
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-
-
-
-
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-
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1
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1
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2
1
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2
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3
1
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-
-
441446
He
Affinity chromatography, subst ...
Medicago sativa
Arch. Biochem. Biophys.
336
121-129
1996
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1
-
4
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1
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2
1
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5
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1
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1
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1
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1
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2
1
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5
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1
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1
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-
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-
-
-
389479
Dalkin
Stress responses in alfalfa (M ...
Medicago sativa
Plant Physiol.
92
440-446
1990
2
-
-
-
-
1
-
-
-
1
1
-
-
4
-
-
1
-
-
1
-
-
5
1
-
-
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-
2
-
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-
2
-
2
-
-
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-
-
1
-
-
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-
1
1
-
-
-
-
1
-
1
-
-
5
1
-
-
-
-
2
-
-
2
1
-
-
1
-
-