BRENDA - Enzyme Database
show all sequences of 2.1.1.15

Calmodulin- and calcium-insensitive fatty acid methyltransferase from RINm5F cells. Inhibition by trifluoperazine and W7

Safayhi, H.; Anazodo, M.I.; Ammon, H.P.T.; Int. J. Biochem. 23, 769-772 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
no effect below 0.5 mM
Rattus norvegicus
additional information
Ca2+, EGTA and calmodulin have no effect
Rattus norvegicus
N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide
W7, very strong inhibition
Rattus norvegicus
S-adenosylhomocysteine
-
Rattus norvegicus
Trifluoperazine
very strong inhibition
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + a fatty acid
Rattus norvegicus
involved in biosynthesis of phosphatidylcholine
S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
insulinoma cell line RINm5F
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
retina
membrane
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000018
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + a fatty acid
-
485161
Rattus norvegicus
S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
485161
Rattus norvegicus
?
S-adenosyl-L-methionine + a fatty acid
involved in biosynthesis of phosphatidylcholine
485161
Rattus norvegicus
S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
485161
Rattus norvegicus
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485161
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
485161
Rattus norvegicus
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
no effect below 0.5 mM
Rattus norvegicus
additional information
Ca2+, EGTA and calmodulin have no effect
Rattus norvegicus
N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide
W7, very strong inhibition
Rattus norvegicus
S-adenosylhomocysteine
-
Rattus norvegicus
Trifluoperazine
very strong inhibition
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + a fatty acid
Rattus norvegicus
involved in biosynthesis of phosphatidylcholine
S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
Rattus norvegicus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
retina
membrane
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.000018
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + a fatty acid
-
485161
Rattus norvegicus
S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
485161
Rattus norvegicus
?
S-adenosyl-L-methionine + a fatty acid
involved in biosynthesis of phosphatidylcholine
485161
Rattus norvegicus
S-adenosyl-L-homocysteine + a fatty acid methyl ester
-
485161
Rattus norvegicus
?
S-adenosyl-L-methionine + phosphatidylethanolamine
-
485161
Rattus norvegicus
S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
-
485161
Rattus norvegicus
?
Other publictions for EC 2.1.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733645
Petronikolou
Biochemical studies of mycobac ...
Mycobacterium marinum, Mycobacterium marinum ATCC BAA-535
Chem. Biol.
22
1480-1490
2015
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1
1
4
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7
-
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5
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8
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7
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1
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1
4
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7
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-
-
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8
-
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7
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-
-
-
-
-
-
-
10
10
718642
Nawabi
Engineering Escherichia coli f ...
Mycobacterium marinum
Appl. Environ. Microbiol.
77
8052-8061
2011
-
-
1
-
-
-
-
6
-
-
-
-
-
2
-
-
1
-
-
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-
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6
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6
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-
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1
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6
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1
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
485162
Sastry
S-Adenosyl-L-methionine-mediat ...
Rattus norvegicus
J. Ocul. Pharmacol.
10
253-263
1994
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-
-
-
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1
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1
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1
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1
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1
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1
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1
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-
-
-
-
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-
485160
Orpiszewski
-
Multiple forms of O-methyltran ...
Mycobacterium sp., Mycolicibacterium phlei
FEMS Microbiol. Lett.
82
233-236
1991
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-
-
-
-
-
-
-
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2
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2
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1
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8
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2
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1
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8
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-
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485161
Safayhi
Calmodulin- and calcium-insens ...
Rattus norvegicus
Int. J. Biochem.
23
769-772
1991
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-
-
-
-
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5
-
-
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-
1
-
2
-
-
-
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1
1
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3
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5
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1
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1
1
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3
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-
485159
Akamatsu
The enzymatic synthesis of fat ...
Mycolicibacterium phlei
J. Biol. Chem.
245
709-713
1970
-
-
-
-
-
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3
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
2
-
1
1
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-
-
-
-
-
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3
-
2
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1
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4
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2
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1
1
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