BRENDA - Enzyme Database show
show all sequences of 2.1.1.148

Functional analysis of the Mycobacterium tuberculosis FAD-dependent thymidylate synthase, ThyX, reveals new amino acid residues contributing to an extended ThyX motif

Ulmer, J.E.; Boum, Y.; Thouvenel, C.D.; Myllykallio, H.; Sibley, C.H.; J. Bacteriol. 190, 2056-2064 (2008)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
analysis of 67 site-directed mutants and identification of the extended motif Y44X(24)H69X(25)R95HRX(87)S105XRYX(90)R199 of amino acids essential to enzyme activity. Residue H69 is the catalytic residue, S105 the nucleophile
Mycobacterium tuberculosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WG57
-
-
Mycobacterium tuberculosis H37Rv
P9WG57
-
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
analysis of 67 site-directed mutants and identification of the extended motif Y44X(24)H69X(25)R95HRX(87)S105XRYX(90)R199 of amino acids essential to enzyme activity. Residue H69 is the catalytic residue, S105 the nucleophile
Mycobacterium tuberculosis
Other publictions for EC 2.1.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733512
Alexandrova
Synthesis and evaluation of C- ...
Mycobacterium tuberculosis
Bioorg. Med. Chem.
23
7131-7137
2015
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733298
Becker
Substrate interaction dynamics ...
Paramecium bursaria Chlorella virus 1
Biochem. J.
459
37-45
2014
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734029
Mishanina
Substrate activation in flavin ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Am. Chem. Soc.
136
10597-10600
2014
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734346
Mathews
Flavin-dependent thymidylate s ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Bioterror Biodef.
Suppl 12
004
2013
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1
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719663
Mishanina
Trapping of an intermediate in ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Am. Chem. Soc.
134
4442-4448
2012
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720985
Zhang
Crystal structure of a flavin- ...
Helicobacter pylori
Protein Pept. Lett.
19
1225-1230
2012
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6
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720992
Wang
Crystal structure and enzymati ...
Helicobacter pylori, Helicobacter pylori SS1
Protein Sci.
20
1398-1410
2011
1
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1
1
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1
2
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1
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1
1
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718969
Kan
Biochemical characterization o ...
no activity in Corynebacterium glutamicum strain NCHU 87078
Biochim. Biophys. Acta
1804
1751-1759
2010
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697906
Wang
Oxidase activity of a flavin-d ...
Thermotoga maritima
FEBS J.
276
2801-2810
2009
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1
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700395
Koehn
An unusual mechanism of thymid ...
Thermotoga maritima
Nature
458
919-923
2009
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1
2
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691285
Esra Onen
Design, synthesis and evaluati ...
Paramecium bursaria Chlorella virus 1
Bioorg. Med. Chem. Lett.
18
3628-3631
2008
-
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5
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1
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1
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1
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5
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5
5
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1
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694812
Hunter
Kinetics and ligand-binding pr ...
Mycobacterium tuberculosis
PLoS ONE
3
e2237
2008
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1
1
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3
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2
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1
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1
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1
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1
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1
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698579
Ulmer
Functional analysis of the Myc ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
190
2056-2064
2008
-
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1
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10
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700990
Escartin
Flavin-dependent thymidylate s ...
Helicobacter pylori, Paramecium bursaria Chlorella virus 1
Proc. Natl. Acad. Sci. USA
105
9948-9952
2008
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4
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1
2
2
1
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671555
Chernyshev
Thymidyl biosynthesis enzymes ...
Bacillus anthracis, Chlamydia trachomatis, Clostridium botulinum, Helicobacter pylori, Mycobacterium leprae, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, no activity in Homo sapiens, Paramecium bursaria Chlorella virus-1, Pyrococcus furiosus, Rickettsia sp., Thermotoga maritima, Treponema palladium
Appl. Microbiol. Biotechnol.
74
282-289
2007
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1
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3
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2
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17
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3
1
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2
3
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3
1
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691759
Chernyshev
The relationships between oxid ...
Thermotoga maritima
Chem. Commun. (Camb. )
27
2861-2863
2007
-
1
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1
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1
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1
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1
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692837
Leduc
Flavin-dependent thymidylate s ...
Rhodobacter capsulatus, Rhodobacter capsulatus MT1131
J. Bacteriol.
189
8537-8545
2007
-
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2
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6
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4
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678023
Kanai
Archaeal Pyrococcus furiosus t ...
Pyrococcus furiosus
Biochem. J.
393
373-379
2006
-
1
1
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1
1
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3
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1
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1
1
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1
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1
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1
1
1
1
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679184
Mason
A lag-phase in the reduction o ...
Thermotoga maritima
Chem. Commun. (Camb. )
16
1781-1783
2006
-
-
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1
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1
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1
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680675
Graziani
Catalytic mechanism and struct ...
Paramecium bursaria Chlorella virus-1
J. Biol. Chem.
281
24048-24057
2006
-
-
1
1
9
-
2
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4
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1
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1
1
1
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2
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1
2
1
9
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2
2
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1
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1
1
1
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681384
Sampathkumar
NADP+ expels both the co-facto ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
360
1-6
2006
-
1
1
1
1
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7
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1
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1
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1
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658992
Gattis
Direct observation of the part ...
Campylobacter jejuni
J. Am. Chem. Soc.
127
832-833
2005
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1
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681375
Sampathkumar
Structure of the Mycobacterium ...
Mycobacterium tuberculosis
J. Mol. Biol.
352
1091-1104
2005
-
1
1
1
11
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7
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1
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1
1
1
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1
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1
1
1
1
11
-
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1
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1
1
1
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657899
Leduc
Two distinct pathways for thym ...
Aeropyrum pernix, Aquifex aeolicus, no activity in Archaeoglobus fulgidus, no activity in Methanocaldococcus jannaschii, no activity in Methanopyrus kandleri, no activity in Methanopyrus kandleri AV19, no activity in Methanothermobacter thermoautotrophicus, Pyrobaculum aerophilum, Pyrococcus abyssi, Pyrococcus furiosus, Pyrococcus horikoshii, Saccharolobus solfataricus, Sulfurisphaera tokodaii, Thermoplasma acidophilum, Thermoplasma volcanium, Thermotoga maritima
Biochem. Soc. Trans.
32
231-235
2004
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17
-
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22
-
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22
-
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-
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658015
Agrawal
Mechanistic studies of a flavi ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Biochemistry
43
10295-10301
2004
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1
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1
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1
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6
-
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1
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2
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1
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2
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1
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1
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1
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659052
Liu
Bacterial thymidylate synthase ...
Trichodesmium erythraeum
J. Bacteriol.
186
6316-6319
2004
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1
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1
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1
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-
659429
Graziani
Functional analysis of FAD-dep ...
Paramecium bursaria Chlorella virus-1
J. Biol. Chem.
279
54340-54347
2004
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-
1
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1
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5
2
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1
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5
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1
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2
1
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-
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3
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1
3
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1
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5
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2
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1
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1
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2
1
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-
660374
Leduc
Functional evidence for active ...
Helicobacter pylori
Proc. Natl. Acad. Sci. USA
101
7252-7257
2004
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-
-
-
10
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1
7
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4
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1
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1
1
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7
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1
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1
-
10
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1
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7
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1
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1
1
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7
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-
637234
Montfort
Complementing thymidylate synt ...
Dictyostelium discoideum, Thermotoga maritima
Structure
11
607-608
2003
4
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-
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2
-
2
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4
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2
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4
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2
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2
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4
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637235
Mathews
Functional analysis of substra ...
Bacillus anthracis, Borreliella burgdorferi, Campylobacter jejuni, Chlamydia pneumoniae, Chlamydia psittaci, Chlamydia trachomatis, Clostridioides difficile, Clostridium botulinum, Clostridium perfringens, Corynebacterium diphtheriae, Dictyostelium discoideum, Ehrlichia chaffeensis, Helicobacter pylori, Mycobacterium avium, Mycobacterium leprae, Mycobacterium tuberculosis, Mycobacterium tuberculosis variant bovis, Rhodococcus sp., Rickettsia conorii, Rickettsia prowazekii, Thermotoga maritima, Thermotoga maritima DSM 3109, Treponema denticola, Treponema pallidum
Structure
11
677-690
2003
6
21
1
1
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-
2
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24
-
26
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1
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-
71
2
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46
-
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6
22
1
46
1
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2
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24
-
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1
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71
2
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-
637232
Myllykallio
An alternative flavin-dependen ...
Borreliella burgdorferi, Campylobacter jejuni, Chlamydia sp., Dictyostelium discoideum, Helicobacter pylori, Mycobacterium tuberculosis, Pyrococcus abyssi, Pyrococcus horikoshii, Rickettsia prowazekii, Saccharolobus solfataricus, Treponema pallidum
Science
297
105-107
2002
3
4
2
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1
-
-
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-
3
11
-
11
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1
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-
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33
1
-
-
-
-
-
-
-
22
-
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-
3
4
2
22
-
1
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-
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-
3
11
-
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1
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-
33
1
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-
-
-
-
-
-
-
-
-
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-
-
637233
Giladi
Genetic evidence for a novel t ...
Campylobacter jejuni, Halobacterium salinarum
FEMS Microbiol. Lett.
216
105-109
2002
-
-
2
-
-
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1
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1
2
-
2
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1
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4
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2
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1
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1
2
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1
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4
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