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Literature summary for 2.1.1.143 extracted from

  • Nes, W.D.; Sinha, A.; Jayasimha, P.; Zhou, W.; Song, Z.; Dennis, A.L.
    Probing the sterol binding site of soybean sterol methyltransferase by site-directed mutagenesis: functional analysis of conserved aromatic amino acids in Region 1 (2006), Arch. Biochem. Biophys., 448, 23-30.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y83F Km-value for 24-methylenelophenol is identical to wild-type value, turnover number is 5fold higher than wild-type value Glycine max
Y83L inactive mutant enzyme Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
24-methylenelophenol wild-type enzyme and mutant enzyme Y83F Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 24-methylenelophenol
-
Glycine max S-adenosyl-L-homocysteine + (Z)-24-ethylidenelophenol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
24-methylenelophenol wild-type enzyme Glycine max
0.005
-
24-methylenelophenol mutant enzyme Y83F Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 7.7
-
Glycine max