Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Glycine max |
expressed in Escherichia coli, critical 24H replaced by a fluorine atome | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24-bromocycloartenol | competitive; competitive inhibitor relative to cycloartenol; time-dependent inhibition kinetics | Glycine max | |
24-fluorocycloartenol | allylic substrate analog, time-dependent inhibition kinetics, consistent with the action of a suicide substrate that irreversibly inactivates the enzyme; competitive, the electron-withdrawing alpha-fluorine substituent suppresses the rate of the C-methylation reaction | Glycine max | |
24-thiacycloartenol | - |
Glycine max | |
26,27-dehydrocycloartenol | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
24-fluorocycloartenol | pH 7.5, 35°C | Glycine max | |
0.033 | - |
24-fluorocycloartenol | - |
Glycine max | |
0.033 | - |
24-fluorocycloartenol | kinetic mechanism shown | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Glycine max | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cycloartenol + S-adenosyl-L-methionine | Glycine max | - |
(24S)-24-methylcycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + cycloartenol | Glycine max | - |
S-adenosyl-L-homocysteine + 24-methylenecycloartanol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Glycine max | - |
isozyme SMT1 | - |
Purification (Comment) | Organism |
---|---|
partially purified | Glycine max |
SDS-PAGE | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | heterologously expressed in Escherichia coli | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
synthesis and kinetic analysis of the fluorinated substrate analog 24-fluorocycloartenol, electron-withdrawing alpha-fluorine substituent suppresses the rate of the C-methylation reaction by one order of magnitude, critical hydride shift of H24 to C25 to afford time-dependent enzyme inactivation prevented by analyzed substrate analog | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24-fluorocycloartenol + S-adenosyl-L-methionine | allylic substrate analog | Glycine max | (24R)-24-methyl-24-fluoro-cycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Glycine max | (24S)-24-methylcycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + 24-fluorocycloartenol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + cycloartenol | - |
Glycine max | S-adenosyl-L-homocysteine + 24-methylenecycloartanol | - |
? | |
S-adenosyl-L-methionine + cycloartenol | fluorine-modified sterol side chains designed to examine the electrophilic nature of the common C-methylation reaction affecting C1 and C2-activities, might retard C-methylation reaction to afford protein alkylation | Glycine max | S-adenosyl-L-homocysteine + 24-methylenecycloartanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMT | - |
Glycine max |
SMT protein | - |
Glycine max |
SMT1 | - |
Glycine max |
sterol 24-C methyltransferase | - |
Glycine max |
sterol C-24 methyltransferase | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Glycine max |
35 | - |
inhibition assay at | Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
24-fluorocycloartenol | pH 7.5, 35°C | Glycine max | |
0.00033 | - |
24-fluorocycloartenol | - |
Glycine max | |
0.01 | - |
Cycloartenol | pH 7.5, 35°C | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Glycine max | |
0.032 | - |
24-fluorocycloartenol | competitive inhibition, concentration of substrate analogs varied at 5, 15, 25, and 50 microM | Glycine max | |
0.032 | - |
24-fluorocycloartenol | pH 7.5, 35°C, versus cycloartenol | Glycine max | |
0.036 | - |
24-bromocycloartenol | - |
Glycine max | |
0.036 | - |
24-bromocycloartenol | competitive inhibition, concentration of substrate analogs varied at 5, 15, 25, and 50 microM | Glycine max | |
0.036 | - |
24-bromocycloartenol | pH 7.5, 35°C, versus cycloartenol | Glycine max |