Cloned (Comment) | Organism |
---|---|
- |
Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24(28)-methylenecycloartanol | competitive with respect to S-adenosyl-L-methionine | Glycine max | |
25-Azacycloartenol | noncompetitive inhibitor versus cycloartenol, uncompetitive inhibitor versus S-adenosyl-L-methionine | Glycine max | |
cyclolaudenol | dead end inhibitor analog, competitive versus cycloartenol, uncompetitive versus S-adenosyl-L-methionine | Glycine max | |
S-adenosyl-L-homocysteine | noncompetitive with respect to S-adenosyl-L-methionine | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
24(28)-methylenelanosterol | - |
Glycine max | |
0.022 | - |
24(28)-methylenecycloartanol | - |
Glycine max | |
0.027 | - |
lanosterol | - |
Glycine max | |
0.03 | - |
Cycloartenol | - |
Glycine max | |
0.03 | - |
24(28)-methylenelophenol | - |
Glycine max | |
0.032 | - |
31-norcycloartenol | - |
Glycine max | |
0.035 | - |
Zymosterol | - |
Glycine max | |
0.043 | - |
parkeol | - |
Glycine max | |
0.045 | - |
24-dehydropollinstanol | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
4 * 41500, SDS-PAGE | Glycine max |
161000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Glycine max |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 24(28)-methylenecycloartanol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 24(28)-methylenelanosterol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 24(28)-methylenelophenol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 24-dehydropollinstanol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 31-norcycloartenol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + cycloartenol | successive C-methylations of the DELTA24 bond occurs at the same active center. The two methylation steps can proceed by a change in chemical mechanism resulting from differences in sterol structure, concerted versus carbocation. The kinetic mechanism remains the same during the consecutive methylation of the DELTA24 bond | Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + lanosterol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + parkeol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + zymosterol | - |
Glycine max | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41500, SDS-PAGE | Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
25-Azacycloartenol | inhibition versus cycloartenol | Glycine max | |
0.0001 | - |
25-Azacycloartenol | inhibition versus S-adenosyl-L-methionine | Glycine max | |
0.007 | - |
cyclolaudenol | inhibition versus cycloartenol | Glycine max | |
0.009 | - |
24(28)-methylene cycloartanol | inhibition versus S-adenosyl-L-methionine | Glycine max | |
0.009 | - |
cyclolaudenol | inhibition versus S-adenosyl-L-methionine | Glycine max | |
0.047 | - |
S-adenosyl-L-homocysteine | inhibition versus cycloartenol | Glycine max | |
0.056 | - |
S-adenosyl-L-homocysteine | inhibition versus S-adenosyl-L-methionine | Glycine max | |
0.171 | - |
24(28)-methylene cycloartanol | inhibition versus cycloartenol | Glycine max |