Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24(28)-methylenecycloartanol | competitive with cycloartenol | Arabidopsis thaliana | |
24beta-methylcycloartanol | competitive with cycloartenol | Arabidopsis thaliana | |
25-Azacycloartenol | competitive with cycloartenol or 24(28)-methylenelophenol; noncompetitive | Arabidopsis thaliana | |
26,27-dehydrozymosterol | mechanism-based inhibitor, specific covalent modification of SMT2 | Arabidopsis thaliana | |
citrostadienol | competitive with cycloartenol or 24(28)-methylenelophenol | Arabidopsis thaliana | |
sitosterol | competitive with cycloartenol or 24(28)-methylenelophenol | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
26,27-dehydrozymosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.016 | - |
fecosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.023 | - |
dehydropollinastanol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.028 | - |
Zymosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.03 | - |
31-norcycloartenol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.03 | - |
24(28)-methylenelophenol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.03 | - |
4alpha-methylfecosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.03 | - |
4alpha-methylzymosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.03 | - |
cholest-7,24-dienol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.035 | - |
Cycloartenol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.035 | - |
24(28)-methylenelanosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.035 | - |
24(28)-methylenecholest-7-enol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.036 | - |
24(28)-methylenecholesterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.038 | - |
desmosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.038 | - |
obtusifoliol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.04 | - |
24(28)-methylenecycloartanol | pH 7.5, 35°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Arabidopsis thaliana |
160000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cycloartenol + S-adenosyl-L-methionine | Arabidopsis thaliana | SMT2 has a position-specific substrate specificity for DELTA24(25)-sterols and contains a single active center to catalyze the consecutive C1-transfer activities by substrate reaction channels similar to the fungal SMT1 | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24(28)-methylenecholest-7-enol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
24(28)-methylenecholesterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
24(28)-methylenecycloartanol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
24(28)-methylenelanosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
24(28)-methylenelophenol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | additional information | a mixture of three stereochemical related products with DELTA24(28)Z-ethylidene, DELTA24(28)-ethylidene and DELTA25(27)-24beta-ethyl side chains | ? | |
26,27-dehydrozymosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | 26-homocholesta-8(9),23(24)E,26(26')-trienol + 26-homocholesta-8(9),26(26')-3beta,24beta-dienol | - |
? | |
31-norcycloartenol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
4alpha-methylfecosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
4alpha-methylzymosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
cholest-7,24-dienol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
cycloartenol + S-adenosyl-L-methionine | SMT2 has a position-specific substrate specificity for DELTA24(25)-sterols and contains a single active center to catalyze the consecutive C1-transfer activities by substrate reaction channels similar to the fungal SMT1 | Arabidopsis thaliana | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
dehydropollinastanol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
desmosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
fecosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with cycloartanol, 3-deoxycycloartenol, or cyclolaudenol | Arabidopsis thaliana | ? | - |
? | |
obtusifoliol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | ? | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
SMT2 | - |
Arabidopsis thaliana |
sterol methyltransferase2 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
26,27-dehydrozymosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.01 | - |
Cycloartenol | pH 7.5, 35°C | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
25-Azacycloartenol | pH 7.5, 35°C, substrate: cycloartenol | Arabidopsis thaliana | |
0.045 | - |
citrostadienol | pH 7.4, 35°C, substrate: 24(28)-methylenelophenol | Arabidopsis thaliana | |
0.049 | - |
26,27-dehydrozymosterol | pH 7.5, 35°C | Arabidopsis thaliana | |
0.05 | - |
citrostadienol | pH 7.4, 35°C, substrate cycloartenol | Arabidopsis thaliana | |
0.15 | - |
24beta-methylcycloartanol | pH 7.4, 35°C, substrate cycloartenol | Arabidopsis thaliana | |
0.2 | - |
sitosterol | pH 7.5, 35°C, substrate: cycloartenol | Arabidopsis thaliana | |
0.207 | - |
sitosterol | pH 7.4, 35°C, substrate: 24(28)-methylenelophenol | Arabidopsis thaliana | |
0.25 | - |
24(28)-methylene cycloartanol | pH 7.4, 35°C, substrate cycloartenol | Arabidopsis thaliana |