Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.142 extracted from

  • Zhou, W.; Nes, W.D.
    Sterol methyltransferase2: purification, properties, and inhibition (2003), Arch. Biochem. Biophys., 420, 18-34.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
24(28)-methylenecycloartanol competitive with cycloartenol Arabidopsis thaliana
24beta-methylcycloartanol competitive with cycloartenol Arabidopsis thaliana
25-Azacycloartenol competitive with cycloartenol or 24(28)-methylenelophenol; noncompetitive Arabidopsis thaliana
26,27-dehydrozymosterol mechanism-based inhibitor, specific covalent modification of SMT2 Arabidopsis thaliana
citrostadienol competitive with cycloartenol or 24(28)-methylenelophenol Arabidopsis thaliana
sitosterol competitive with cycloartenol or 24(28)-methylenelophenol Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
26,27-dehydrozymosterol pH 7.5, 35°C Arabidopsis thaliana
0.016
-
fecosterol pH 7.5, 35°C Arabidopsis thaliana
0.023
-
dehydropollinastanol pH 7.5, 35°C Arabidopsis thaliana
0.028
-
Zymosterol pH 7.5, 35°C Arabidopsis thaliana
0.03
-
31-norcycloartenol pH 7.5, 35°C Arabidopsis thaliana
0.03
-
24(28)-methylenelophenol pH 7.5, 35°C Arabidopsis thaliana
0.03
-
4alpha-methylfecosterol pH 7.5, 35°C Arabidopsis thaliana
0.03
-
4alpha-methylzymosterol pH 7.5, 35°C Arabidopsis thaliana
0.03
-
cholest-7,24-dienol pH 7.5, 35°C Arabidopsis thaliana
0.035
-
Cycloartenol pH 7.5, 35°C Arabidopsis thaliana
0.035
-
24(28)-methylenelanosterol pH 7.5, 35°C Arabidopsis thaliana
0.035
-
24(28)-methylenecholest-7-enol pH 7.5, 35°C Arabidopsis thaliana
0.036
-
24(28)-methylenecholesterol pH 7.5, 35°C Arabidopsis thaliana
0.038
-
desmosterol pH 7.5, 35°C Arabidopsis thaliana
0.038
-
obtusifoliol pH 7.5, 35°C Arabidopsis thaliana
0.04
-
24(28)-methylenecycloartanol pH 7.5, 35°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Arabidopsis thaliana
160000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cycloartenol + S-adenosyl-L-methionine Arabidopsis thaliana SMT2 has a position-specific substrate specificity for DELTA24(25)-sterols and contains a single active center to catalyze the consecutive C1-transfer activities by substrate reaction channels similar to the fungal SMT1 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24(28)-methylenecholest-7-enol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
24(28)-methylenecholesterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
24(28)-methylenecycloartanol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
24(28)-methylenelanosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
24(28)-methylenelophenol + S-adenosyl-L-methionine
-
Arabidopsis thaliana additional information a mixture of three stereochemical related products with DELTA24(28)Z-ethylidene, DELTA24(28)-ethylidene and DELTA25(27)-24beta-ethyl side chains ?
26,27-dehydrozymosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana 26-homocholesta-8(9),23(24)E,26(26')-trienol + 26-homocholesta-8(9),26(26')-3beta,24beta-dienol
-
?
31-norcycloartenol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
4alpha-methylfecosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
4alpha-methylzymosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
cholest-7,24-dienol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
cycloartenol + S-adenosyl-L-methionine
-
Arabidopsis thaliana 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
?
cycloartenol + S-adenosyl-L-methionine SMT2 has a position-specific substrate specificity for DELTA24(25)-sterols and contains a single active center to catalyze the consecutive C1-transfer activities by substrate reaction channels similar to the fungal SMT1 Arabidopsis thaliana 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
?
dehydropollinastanol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
desmosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
fecosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
additional information no activity with cycloartanol, 3-deoxycycloartenol, or cyclolaudenol Arabidopsis thaliana ?
-
?
obtusifoliol + S-adenosyl-L-methionine
-
Arabidopsis thaliana ?
-
?
zymosterol + S-adenosyl-L-methionine
-
Arabidopsis thaliana fecosterol + S-adenosyl-L-homocysteine
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
SMT2
-
Arabidopsis thaliana
sterol methyltransferase2
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
26,27-dehydrozymosterol pH 7.5, 35°C Arabidopsis thaliana
0.01
-
Cycloartenol pH 7.5, 35°C Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
25-Azacycloartenol pH 7.5, 35°C, substrate: cycloartenol Arabidopsis thaliana
0.045
-
citrostadienol pH 7.4, 35°C, substrate: 24(28)-methylenelophenol Arabidopsis thaliana
0.049
-
26,27-dehydrozymosterol pH 7.5, 35°C Arabidopsis thaliana
0.05
-
citrostadienol pH 7.4, 35°C, substrate cycloartenol Arabidopsis thaliana
0.15
-
24beta-methylcycloartanol pH 7.4, 35°C, substrate cycloartenol Arabidopsis thaliana
0.2
-
sitosterol pH 7.5, 35°C, substrate: cycloartenol Arabidopsis thaliana
0.207
-
sitosterol pH 7.4, 35°C, substrate: 24(28)-methylenelophenol Arabidopsis thaliana
0.25
-
24(28)-methylene cycloartanol pH 7.4, 35°C, substrate cycloartenol Arabidopsis thaliana