Application | Comment | Organism |
---|---|---|
synthesis | rational drug target design based on structure-function-relation | Prototheca wickerhamii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24(28)-methylenecycloartenol | - |
Prototheca wickerhamii | |
25-Azacycloartenol | - |
Prototheca wickerhamii | |
cyclolaudanol | - |
Prototheca wickerhamii | |
ergosterol | - |
Prototheca wickerhamii | |
additional information | non-competitive inhibitors are ammonium group substituted for carbon at C25, C24, C23 and C22; overview, diverse substrate and transition state analogues | Prototheca wickerhamii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview | Prototheca wickerhamii | |
0.028 | - |
Cycloartenol | - |
Prototheca wickerhamii | |
0.03 | - |
Cycloartenol | - |
Prototheca wickerhamii | |
0.038 | - |
Zymosterol | - |
Prototheca wickerhamii | |
0.063 | - |
desmosterol | - |
Prototheca wickerhamii | |
0.1 | - |
cholesta-5,20(22)E,24-trienol | - |
Prototheca wickerhamii | |
0.112 | - |
cholesta-5,7,22E,24-tetraenol | - |
Prototheca wickerhamii | |
0.125 | - |
14alpha-methylzymosterol | - |
Prototheca wickerhamii | |
0.176 | - |
lanosterol | - |
Prototheca wickerhamii | |
0.276 | - |
cholesta-5,20(22)E,24-trienol | - |
Prototheca wickerhamii | |
0.61 | - |
31-norcycloartenol | - |
Prototheca wickerhamii | |
0.8 | - |
parkeol | - |
Prototheca wickerhamii | |
0.97 | - |
30,31-dinorcycloartenol | - |
Prototheca wickerhamii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Prototheca wickerhamii | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
154000 | - |
gel filtration | Prototheca wickerhamii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cycloartenol | Prototheca wickerhamii | - |
S-adenosyl-L-homocysteine + (24R)-24-methylcycloart-25-en-3beta-ol | - |
? | |
S-adenosyl-L-methionine + cycloartenol | Prototheca wickerhamii | - |
S-adenosyl-L-homocysteine + cyclolaudenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prototheca wickerhamii | - |
- |
- |
Prototheca wickerhamii | - |
algae | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + cycloartenol = S-adenosyl-L-homocysteine + cyclolaudenol | mechanism | Prototheca wickerhamii | |
S-adenosyl-L-methionine + cycloartenol = S-adenosyl-L-homocysteine + cyclolaudenol | structure-function relationship | Prototheca wickerhamii | |
S-adenosyl-L-methionine + cycloartenol = S-adenosyl-L-homocysteine + cyclolaudenol | mechanism, S-adenosyl-L-methionine mehtylates the Si face of the 24(25)-double bond with elimination of a hydrogen atom from the pro-Z methyl group at C-25 | Prototheca wickerhamii | |
S-adenosyl-L-methionine + cycloartenol = S-adenosyl-L-homocysteine + cyclolaudenol | kinetic and inhibition mechanism | Prototheca wickerhamii | |
S-adenosyl-L-methionine + cycloartenol = S-adenosyl-L-homocysteine + cyclolaudenol | steric substrate preferences: 3beta-hydroxy group, planar nucleus, side chain orientated into right-handed structure | Prototheca wickerhamii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cycloartenol + S-adenosyl-L-methionine | - |
Prototheca wickerhamii | 24-methylenecycloartanol + S-adenosyl-L-homocysteine | - |
? | |
desmosterol + S-adenosyl-L-methionine | - |
Prototheca wickerhamii | ? | - |
? | |
lanosterol + S-adenosyl-L-methionine | regiospecific | Prototheca wickerhamii | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + 14alpha-methylzymosterol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 30,31-dinorcycloartenol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 31-norcycloartenol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + cholesta-5,20(22)E,24-trienol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + cholesta-5,7,22E,24-tetraenol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + cycloartenol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + (24R)-24-methylcycloart-25-en-3beta-ol | - |
? | |
S-adenosyl-L-methionine + cycloartenol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + cyclolaudenol | - |
? | |
S-adenosyl-L-methionine + lanosterol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + parkeol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + zymosterol | - |
Prototheca wickerhamii | S-adenosyl-L-homocysteine + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Prototheca wickerhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Prototheca wickerhamii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview, diverse substrate and transition state analogues | Prototheca wickerhamii | |
0.000002 | - |
25-Azacycloartenol | - |
Prototheca wickerhamii | |
0.023 | - |
cyclolaudanol | - |
Prototheca wickerhamii | |
0.08 | - |
ergosterol | - |
Prototheca wickerhamii | |
0.085 | - |
24(28)-methylenecycloartenol | - |
Prototheca wickerhamii |