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Literature summary for 2.1.1.141 extracted from

  • Zhao, N.; Yao, J.; Chaiprasongsuk, M.; Li, G.; Guan, J.; Tschaplinski, T.J.; Guo, H.; Chen, F.
    Molecular and biochemical characterization of the jasmonic acid methyltransferase gene from black cottonwood (Populus trichocarpa) (2013), Phytochemistry, 94, 74-81.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
N361S the mutation leads to higher specific activity with benzoate than with jasmonate Populus trichocarpa
S153Y the mutation leads to strongly reduced activity with benzoate or jasmonate Populus trichocarpa
S153Y/N361S the mutation leads to strongly reduced activity with benzoate or jasmonate Populus trichocarpa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM Ca2+ shows a mild inhibition effect on enzyme activity (about 80% residual activity) Populus trichocarpa
Cu2+ complete inhibition at 5 mM Populus trichocarpa
Fe2+ complete inhibition at 5 mM Populus trichocarpa
Fe3+ complete inhibition at 5 mM Populus trichocarpa
Mg2+ shows a mild inhibition effect on enzyme activity (about 85% residual activity) Populus trichocarpa
Mn2+ 5 mM Mn2+ decreases the enzyme activity by 40% Populus trichocarpa
Zn2+ complete inhibition at 5 mM Populus trichocarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0631
-
benzoate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa
0.175
-
jasmonate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa
0.31
-
jasmonate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa
0.341
-
benzoate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa

Metals/Ions

Metals/Ions Comment Organism Structure
K+ significantly stimulates enzyme activity (to about 250%) Populus trichocarpa
Na+ has a mild stimulation on enzyme activity (to about 160%) Populus trichocarpa
NH4+ has a mild stimulation on enzyme activity (to about 165%) Populus trichocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + jasmonate Populus trichocarpa
-
S-adenosyl-L-homocysteine + methyl jasmonate
-
?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa B9H4E1 black cottonwood
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Populus trichocarpa
-
seedling
-
Populus trichocarpa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with salicylic acid, indole-3-acetic acid, and gibberellic acid Populus trichocarpa ?
-
?
S-adenosyl-L-methionine + benzoate 22% activity compared to jasmonate Populus trichocarpa S-adenosyl-L-homocysteine + methyl benzoate
-
?
S-adenosyl-L-methionine + jasmonate
-
Populus trichocarpa S-adenosyl-L-homocysteine + methyl jasmonate
-
?

Synonyms

Synonyms Comment Organism
jasmonic acid methyltransferase
-
Populus trichocarpa
JMT1
-
Populus trichocarpa
S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase
-
Populus trichocarpa
SABATH3 formerly Populus trichocarpa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 60 the enzyme is 100% stable for 30 min at 4°C. It loses about 10%, 20% and 40% of its maximal activity when incubated at 37°C, 42°C and 50°C, respectively for 30 min. When incubated at 60°C for 30 min, the enzyme loses all of its activity Populus trichocarpa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
benzoate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa
0.013
-
jasmonate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa
0.015
-
jasmonate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa
0.028
-
benzoate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Populus trichocarpa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 at pH 6.0, pH 7.0, pH 8.0 and pH 8.5, the enzyme displays about 80%, 50%, 20% and 5% of its maximal activity, respectively Populus trichocarpa

Expression

Organism Comment Expression
Populus trichocarpa the enzyme expression is induced by methyl jasmonate and salicylic acid as well as alamethicin up

General Information

General Information Comment Organism
physiological function the enzyme plays a role in plant defense against biotic stresses Populus trichocarpa