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Literature summary for 2.1.1.140 extracted from

  • Desgagne-Penix, I.; Facchini, P.
    Systematic silencing of benzylisoquinoline alkaloid biosynthetic genes reveals the major route to papaverine in opium poppy (2012), Plant J., 72, 331-344.
    View publication on PubMed
Search for more literature for 2.1.1.140

Cloned(Commentary)

Cloned (Comment) Organism
real-time quantitative PCR analysis of target gene transcript levels, comparison of target gene transcript levels and papaverine content in individual opium poppy plants infiltrated with Agrobacterium tumefaciens harboring control and gene-specific pTRV2 constructs Papaver somniferum

Protein Variants

Protein Variants Comment Organism
additional information virus-induced gene silencing in opium poppy to functionally characterize six genes potentially involved in papaverine biosynthesis, e.g. the gene encoding coclaurine N-methyltransferase, altered alkaloid content phenotypes, overview. Norlaudanine is only detected in CNMT-suppressed plants Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (S)-coclaurine Papaver somniferum
-
 S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Organism

Organism UniProt Comment Textmining
Papaver somniferum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (S)-coclaurine
-
 Papaver somniferum S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Synonyms

Synonyms Comment Organism
6OMT
-
 Papaver somniferum
CNMT
-
 Papaver somniferum
coclaurine N-methyltransferase
-
 Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Papaver somniferum

General Information

General Information Comment Organism
malfunction plants exhibiting reduced CNMT transcript levels show a significant fourfold increase in the relative abundance of papaverine, and a reduction in the accumulation of thebaine and noscapine. Norlaudanine is only detected in CNMT-suppressed plants. The levels of several N-methylated compounds, including N-methylcoclaurine, N,N-dimethylcoclaurine, 4'-O-methyl-N-methylcoclaurine, reticuline, N,N-dimethylreticuline, codamine, laudanine, laudanosine and salutaridine, and four phathelideisoquinoline alkaloids (i.e. narcotoline, noscapine, N,N-dimethylnarcotine and another noscapine-like compound) are also reduced in CNMT-suppressed plants Papaver somniferum
metabolism the enzyme is involved in the biosynthesis pathways of alkaloids in Papaver somniferum, overview. Subsequent 6-O-methylation, N-methylation, 3'-hydroxylation and 4'-O-methylation convert (S)-norcoclaurine to (S)-reticuline, which is a branch-point intermediate in the formation of most BIA structural sub-groups, including morphinan (e.g. morphine), phthalideisoquinoline (e.g. noscapine) and benzophenanthridine (e.g. sanguinarine) alkaloids Papaver somniferum