Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-methyltetrahydropteroyl-alpha-glutamate | - |
Escherichia coli | |
EDTA | in absence of Mg2+ | Escherichia coli | |
high ionic strength | - |
Escherichia coli | |
Pteroyl-alpha-glutamylglutamic acid | - |
Escherichia coli | |
Pteroyl-gamma-glutamyl-gamma-glutamylglutamic acid | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
5-methyltetrahydropteroyltriglutamate | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Escherichia coli | |
Mg2+ | at low phosphate concentration, 0.1 mM, the Mg2+-stimulation is 4.5fold, smaller stimulation at higher concentrations of phosphate | Escherichia coli | |
phosphate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50800 | - |
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl | Escherichia coli |
84000 | - |
equilibrium ultracentrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine | - |
Escherichia coli | tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine | - |
? | |
additional information | 5-methyltetrahydropteroyl-alpha-glutamate or 5-methyl-tetrahydropteroyl-alpha-glutamylglutamate cannot replace the triglutamate folate derivative as methyl donor | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.8 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | vitamin-B12 independent enzyme | Escherichia coli |