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Literature summary for 2.1.1.14 extracted from

  • Whitefield, C.D.; Steers, E.J.; Weissbach, H.
    Purification and properties of 5-methyltetrahydropteroyltriglutamate-homocysteine transmethylase (1970), J. Biol. Chem., 245, 390-401.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-methyltetrahydropteroyl-alpha-glutamate
-
Escherichia coli
EDTA in absence of Mg2+ Escherichia coli
high ionic strength
-
Escherichia coli
Pteroyl-alpha-glutamylglutamic acid
-
Escherichia coli
Pteroyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
5-methyltetrahydropteroyltriglutamate
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Escherichia coli
Mg2+ at low phosphate concentration, 0.1 mM, the Mg2+-stimulation is 4.5fold, smaller stimulation at higher concentrations of phosphate Escherichia coli
phosphate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50800
-
x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl Escherichia coli
84000
-
equilibrium ultracentrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
Escherichia coli tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
?
additional information 5-methyltetrahydropteroyl-alpha-glutamate or 5-methyl-tetrahydropteroyl-alpha-glutamylglutamate cannot replace the triglutamate folate derivative as methyl donor Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 50800, equilibrium sedimentation of carboxymethylated enzyme in 5 M guanidine-HCl Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information vitamin-B12 independent enzyme Escherichia coli