Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | As(III) SAM methyltransferase conducts three successive rounds of oxidative methylation, with the glutathione conjugates serving as metalloid donors to the enzyme | Cyanidioschyzon sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cyanidioschyzon sp. |
Protein Variants | Comment | Organism |
---|---|---|
C174A | the mutation leads to loss of As(III) methylation | Cyanidioschyzon sp. |
C224A | the mutation leads to loss of As(III) methylation | Cyanidioschyzon sp. |
C72A | the mutation leads to loss of As(III) methylation, but still shows trivalent methylarsenate methylation | Cyanidioschyzon sp. |
Y70W | the mutant exhibits metalloid binding | Cyanidioschyzon sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyanidioschyzon sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Cyanidioschyzon sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + arsenite | the first product, methylarsonate, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsinate, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine | Cyanidioschyzon sp. | S-adenosyl-L-homocysteine + methylarsonate + dimethylarsinate + trimethylarsine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ArsM | - |
Cyanidioschyzon sp. |
As(III) S-adenosylmethionine methyltransferase | - |
Cyanidioschyzon sp. |
As(III) SAM methyltransferase | - |
Cyanidioschyzon sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Cyanidioschyzon sp. |