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show all sequences of 2.1.1.136

Characterization of a novel 2,4,6-trichlorophenol-inducible gene encoding chlorophenol O-methyltransferase from Trichoderma longibrachiatum responsible for the formation of chloroanisoles and detoxification of chlorophenols

Feltrer, R.; Alvarez-Rodriguez, M.L.; Barreiro, C.; Godio, R.P.; Coque, J.J.; Fungal Genet. Biol. 47, 458-467 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Trichoderma longibrachiatum
Engineering
Amino acid exchange
Commentary
Organism
additional information
partial silencing of cmt1 gene results in a 48.9% decrease of enzyme activity levels in a Trichoderma longibrachiatum At37 transformant strain by comparison with the wild type
Trichoderma longibrachiatum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52400
-
x * 52400, deduced from amino acid sequence
Trichoderma longibrachiatum
65400
-
x * 65400, fusion protein SUMO-CMT1, SDS-PAGE
Trichoderma longibrachiatum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trichoderma longibrachiatum
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE column chromatography. hydroxyapatite column chromatography, Resource Q column chromatography, ultrafiltration, and Superdex 200 gel filtration
Trichoderma longibrachiatum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Trichoderma longibrachiatum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 2,4,6-trichlorophenol
-
719544
Trichoderma longibrachiatum
S-adenosyl-L-homocysteine + 2,4,6-trichloroanisole
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 52400, deduced from amino acid sequence; x * 65400, fusion protein SUMO-CMT1, SDS-PAGE
Trichoderma longibrachiatum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Trichoderma longibrachiatum
deduced from amino acid sequence
-
5.9
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Trichoderma longibrachiatum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
partial silencing of cmt1 gene results in a 48.9% decrease of enzyme activity levels in a Trichoderma longibrachiatum At37 transformant strain by comparison with the wild type
Trichoderma longibrachiatum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52400
-
x * 52400, deduced from amino acid sequence
Trichoderma longibrachiatum
65400
-
x * 65400, fusion protein SUMO-CMT1, SDS-PAGE
Trichoderma longibrachiatum
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE column chromatography. hydroxyapatite column chromatography, Resource Q column chromatography, ultrafiltration, and Superdex 200 gel filtration
Trichoderma longibrachiatum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Trichoderma longibrachiatum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + 2,4,6-trichlorophenol
-
719544
Trichoderma longibrachiatum
S-adenosyl-L-homocysteine + 2,4,6-trichloroanisole
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 52400, deduced from amino acid sequence; x * 65400, fusion protein SUMO-CMT1, SDS-PAGE
Trichoderma longibrachiatum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Trichoderma longibrachiatum
deduced from amino acid sequence
-
5.9
Expression
Organism
Commentary
Expression
Trichoderma longibrachiatum
the expression of the enzyme is induced at transcriptional level by exposure of fungal mycelia to 0.01 mg/ml 2,4,6-trichlorophenol
up
General Information
General Information
Commentary
Organism
physiological function
the enzyme is responsible for the detoxification of chlorophenolic pesticides by O-methylation which results in the formation of chloroanisoles
Trichoderma longibrachiatum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is responsible for the detoxification of chlorophenolic pesticides by O-methylation which results in the formation of chloroanisoles
Trichoderma longibrachiatum
Expression (protein specific)
Organism
Commentary
Expression
Trichoderma longibrachiatum
the expression of the enzyme is induced at transcriptional level by exposure of fungal mycelia to 0.01 mg/ml 2,4,6-trichlorophenol
up
Other publictions for EC 2.1.1.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719544
Feltrer
Characterization of a novel 2, ...
Trichoderma longibrachiatum
Fungal Genet. Biol.
47
458-467
2010
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2
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1
1
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1
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657589
Coque
Characterization of an inducib ...
Trichoderma longibrachiatum, Trichoderma longibrachiatum CECT 20431
Appl. Environ. Microbiol.
69
5089-5095
2003
4
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1
6
2
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2
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4
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1
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1
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19
1
1
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1
1
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1
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4
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1
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6
1
2
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2
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1
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2
1
1
19
1
1
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1
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1
1
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1
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288647
Kikuchi
-
Halogenated phenol O-methyltra ...
Trichoderma sp. 'virgatum'
Jpn. Kokai Tokkyo Koho
8
1996
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