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show all sequences of 2.1.1.136

Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines

Coque, J.J.; Alvarez-Rodriguez, M.L.; Larriba, G.; Appl. Environ. Microbiol. 69, 5089-5095 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
5-methyltetrahydrofolic acid
1 mM, activation to 119% of control
Trichoderma longibrachiatum
DTT
1 mM + 1 mM PMSF, activation to 147% of control
Trichoderma longibrachiatum
glycerol
10%, activation to 122% of control
Trichoderma longibrachiatum
PMSF
1 mM + 1 mM DTT, activation to 147% of control
Trichoderma longibrachiatum
General Stability
General Stability
Organism
freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity
Trichoderma longibrachiatum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
1 mM, 94% inhibition
Trichoderma longibrachiatum
Ca2+
1 mM, 16% inhibition
Trichoderma longibrachiatum
Cu2+
1 mM, 96% inhibition
Trichoderma longibrachiatum
Hg2+
0.1 mM, 96% inhibition
Trichoderma longibrachiatum
S-adenosyl-L-homocysteine
competitive
Trichoderma longibrachiatum
Zn2+
0.1 mM,82% inhibition
Trichoderma longibrachiatum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1359
-
2,4,6-Trichlorophenol
-
Trichoderma longibrachiatum
0.284
-
S-adenosyl-L-methionine
-
Trichoderma longibrachiatum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52500
-
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
112000
-
gel filtration
Trichoderma longibrachiatum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum CECT 20431
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trichoderma longibrachiatum
-
CECT 20431
-
Trichoderma longibrachiatum CECT 20431
-
CECT 20431
-
Purification (Commentary)
Commentary
Organism
-
Trichoderma longibrachiatum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Trichoderma longibrachiatum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00055
-
-
Trichoderma longibrachiatum
Storage Stability
Storage Stability
Organism
-20°C, 10% glycerol, stable for up to 3 months
Trichoderma longibrachiatum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3-dichlorophenol + S-adenosyl-L-methionine
22.5% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,5-trichlorophenol + S-adenosyl-L-methionine
64.2% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,5-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-tribromophenol + S-adenosyl-L-methionine
13% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-tribromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4-dibromophenol + S-adenosyl-L-methionine
36.8% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4-dibromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4-dichlorophenol + S-adenosyl-L-methionine
-
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,5-dichlorophenol + S-adenosyl-L-methionine
19.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,5-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,6-dichlorophenol + S-adenosyl-L-methionine
10.4% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,6-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2-chlorophenol + S-adenosyl-L-methionine
9.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2-chloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
3,4-dichlorophenol + S-adenosyl-L-methionine
9.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
3,4-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
-
Trichoderma longibrachiatum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
42
-
54% loss of activity after 30 min, 89% loss of activity after 1 h
Trichoderma longibrachiatum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
8.5
-
Trichoderma longibrachiatum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8.5
pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum
Trichoderma longibrachiatum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.3789
-
S-adenosyl-L-homocysteine
-
Trichoderma longibrachiatum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Trichoderma longibrachiatum
chromatofocusing pH-range 4-7
-
4.9
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
5-methyltetrahydrofolic acid
1 mM, activation to 119% of control
Trichoderma longibrachiatum
DTT
1 mM + 1 mM PMSF, activation to 147% of control
Trichoderma longibrachiatum
glycerol
10%, activation to 122% of control
Trichoderma longibrachiatum
PMSF
1 mM + 1 mM DTT, activation to 147% of control
Trichoderma longibrachiatum
General Stability (protein specific)
General Stability
Organism
freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity
Trichoderma longibrachiatum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
1 mM, 94% inhibition
Trichoderma longibrachiatum
Ca2+
1 mM, 16% inhibition
Trichoderma longibrachiatum
Cu2+
1 mM, 96% inhibition
Trichoderma longibrachiatum
Hg2+
0.1 mM, 96% inhibition
Trichoderma longibrachiatum
S-adenosyl-L-homocysteine
competitive
Trichoderma longibrachiatum
Zn2+
0.1 mM,82% inhibition
Trichoderma longibrachiatum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.3789
-
S-adenosyl-L-homocysteine
-
Trichoderma longibrachiatum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1359
-
2,4,6-Trichlorophenol
-
Trichoderma longibrachiatum
0.284
-
S-adenosyl-L-methionine
-
Trichoderma longibrachiatum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52500
-
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
112000
-
gel filtration
Trichoderma longibrachiatum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum CECT 20431
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Trichoderma longibrachiatum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Trichoderma longibrachiatum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00055
-
-
Trichoderma longibrachiatum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10% glycerol, stable for up to 3 months
Trichoderma longibrachiatum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,3-dichlorophenol + S-adenosyl-L-methionine
22.5% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,5-trichlorophenol + S-adenosyl-L-methionine
64.2% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,5-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-tribromophenol + S-adenosyl-L-methionine
13% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-tribromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4-dibromophenol + S-adenosyl-L-methionine
36.8% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4-dibromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,4-dichlorophenol + S-adenosyl-L-methionine
-
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,5-dichlorophenol + S-adenosyl-L-methionine
19.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,5-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2,6-dichlorophenol + S-adenosyl-L-methionine
10.4% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,6-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2-chlorophenol + S-adenosyl-L-methionine
9.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2-chloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
3,4-dichlorophenol + S-adenosyl-L-methionine
9.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
3,4-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
-
Trichoderma longibrachiatum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
42
-
54% loss of activity after 30 min, 89% loss of activity after 1 h
Trichoderma longibrachiatum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
8.5
-
Trichoderma longibrachiatum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8.5
pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum
Trichoderma longibrachiatum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Trichoderma longibrachiatum
chromatofocusing pH-range 4-7
-
4.9
Other publictions for EC 2.1.1.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719544
Feltrer
Characterization of a novel 2, ...
Trichoderma longibrachiatum
Fungal Genet. Biol.
47
458-467
2010
-
-
1
-
1
-
-
-
-
-
2
-
-
5
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
1
1
1
-
-
657589
Coque
Characterization of an inducib ...
Trichoderma longibrachiatum, Trichoderma longibrachiatum CECT 20431
Appl. Environ. Microbiol.
69
5089-5095
2003
4
-
-
-
-
1
6
2
-
-
2
2
-
4
-
-
1
-
-
2
1
1
19
1
1
-
1
-
1
1
-
-
1
1
-
4
-
-
-
-
-
1
-
6
1
2
-
-
2
2
-
-
-
1
-
2
1
1
19
1
1
-
1
-
1
1
-
1
-
-
-
-
-
-
288647
Kikuchi
-
Halogenated phenol O-methyltra ...
Trichoderma sp. 'virgatum'
Jpn. Kokai Tokkyo Koho
8
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
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