Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-methyltetrahydrofolic acid | 1 mM, activation to 119% of control | Trichoderma longibrachiatum | |
DTT | 1 mM + 1 mM PMSF, activation to 147% of control | Trichoderma longibrachiatum | |
glycerol | 10%, activation to 122% of control | Trichoderma longibrachiatum | |
PMSF | 1 mM + 1 mM DTT, activation to 147% of control | Trichoderma longibrachiatum |
General Stability | Organism |
---|---|
freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity | Trichoderma longibrachiatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 94% inhibition | Trichoderma longibrachiatum | |
Ca2+ | 1 mM, 16% inhibition | Trichoderma longibrachiatum | |
Cu2+ | 1 mM, 96% inhibition | Trichoderma longibrachiatum | |
Hg2+ | 0.1 mM, 96% inhibition | Trichoderma longibrachiatum | |
S-adenosyl-L-homocysteine | competitive | Trichoderma longibrachiatum | |
Zn2+ | 0.1 mM,82% inhibition | Trichoderma longibrachiatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1359 | - |
2,4,6-Trichlorophenol | - |
Trichoderma longibrachiatum | |
0.284 | - |
S-adenosyl-L-methionine | - |
Trichoderma longibrachiatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
2 * 52500, SDS-PAGE | Trichoderma longibrachiatum |
112000 | - |
gel filtration | Trichoderma longibrachiatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trichlorophenol + S-adenosyl-L-methionine | Trichoderma longibrachiatum | activity is induced by 2,4,6-trichlorophenol | 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,6-trichlorophenol + S-adenosyl-L-methionine | Trichoderma longibrachiatum CECT 20431 | activity is induced by 2,4,6-trichlorophenol | 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma longibrachiatum | - |
CECT 20431 | - |
Trichoderma longibrachiatum CECT 20431 | - |
CECT 20431 | - |
Purification (Comment) | Organism |
---|---|
- |
Trichoderma longibrachiatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trichoderma longibrachiatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00055 | - |
- |
Trichoderma longibrachiatum |
Storage Stability | Organism |
---|---|
-20°C, 10% glycerol, stable for up to 3 months | Trichoderma longibrachiatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine | 10.7% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine | 10.7% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum CECT 20431 | 2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,3,4-trichlorophenol + S-adenosyl-L-methionine | 93.3% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,3,4-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,3,4-trichlorophenol + S-adenosyl-L-methionine | 93.3% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum CECT 20431 | 2,3,4-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,3,6-trichlorophenol + S-adenosyl-L-methionine | 55.6% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,3,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,3,6-trichlorophenol + S-adenosyl-L-methionine | 55.6% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum CECT 20431 | 2,3,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,3-dichlorophenol + S-adenosyl-L-methionine | 22.5% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,3-dichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,5-trichlorophenol + S-adenosyl-L-methionine | 64.2% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,4,5-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,6-tribromophenol + S-adenosyl-L-methionine | 13% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,4,6-tribromoanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,6-trichlorophenol + S-adenosyl-L-methionine | activity is induced by 2,4,6-trichlorophenol | Trichoderma longibrachiatum | 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,6-trichlorophenol + S-adenosyl-L-methionine | 60% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,6-trichlorophenol + S-adenosyl-L-methionine | activity is induced by 2,4,6-trichlorophenol | Trichoderma longibrachiatum CECT 20431 | 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4,6-trichlorophenol + S-adenosyl-L-methionine | 60% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum CECT 20431 | 2,4,6-trichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4-dibromophenol + S-adenosyl-L-methionine | 36.8% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,4-dibromoanisole + S-adenosyl-L-homocysteine | - |
? | |
2,4-dichlorophenol + S-adenosyl-L-methionine | - |
Trichoderma longibrachiatum | 2,3-dichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,5-dichlorophenol + S-adenosyl-L-methionine | 19.6% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,5-dichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2,6-dichlorophenol + S-adenosyl-L-methionine | 10.4% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2,6-dichloroanisole + S-adenosyl-L-homocysteine | - |
? | |
2-chlorophenol + S-adenosyl-L-methionine | 9.6% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 2-chloroanisole + S-adenosyl-L-homocysteine | - |
? | |
3,4-dichlorophenol + S-adenosyl-L-methionine | 9.3% of the O-methylation activity with 2,3-dichlorophenol | Trichoderma longibrachiatum | 3,4-dichloroanisole + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52500, SDS-PAGE | Trichoderma longibrachiatum |
Synonyms | Comment | Organism |
---|---|---|
CPOMT | - |
Trichoderma longibrachiatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Trichoderma longibrachiatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
54% loss of activity after 30 min, 89% loss of activity after 1 h | Trichoderma longibrachiatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.5 | - |
Trichoderma longibrachiatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum | Trichoderma longibrachiatum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3789 | - |
S-adenosyl-L-homocysteine | - |
Trichoderma longibrachiatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichoderma longibrachiatum | chromatofocusing pH-range 4-7 | - |
4.9 |