BRENDA - Enzyme Database show
show all sequences of 2.1.1.133

Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans

Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 175, 7430-7440 (1993)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
x * 31000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-Adenosyl-L-methionine + precorrin-4
Pseudomonas denitrificans (nomen rejiciendum)
enzyme in the biosynthesis of vitamin B12
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
P21922
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + precorrin-4
-
15270
Pseudomonas denitrificans (nomen rejiciendum)
S-adenosyl-L-homocysteine + precorrin-5
-
15270
Pseudomonas denitrificans (nomen rejiciendum)
-
S-Adenosyl-L-methionine + precorrin-4
enzyme in the biosynthesis of vitamin B12
15270
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
x * 31000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
x * 31000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-Adenosyl-L-methionine + precorrin-4
Pseudomonas denitrificans (nomen rejiciendum)
enzyme in the biosynthesis of vitamin B12
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + precorrin-4
-
15270
Pseudomonas denitrificans (nomen rejiciendum)
S-adenosyl-L-homocysteine + precorrin-5
-
15270
Pseudomonas denitrificans (nomen rejiciendum)
-
S-Adenosyl-L-methionine + precorrin-4
enzyme in the biosynthesis of vitamin B12
15270
Pseudomonas denitrificans (nomen rejiciendum)
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
x * 31000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Other publictions for EC 2.1.1.133
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
3209
Roessner
Overexpression in Escherichia ...
Pseudomonas denitrificans (nomen rejiciendum)
Protein Expr. Purif.
6
155-163
1995
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
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2
1
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-
-
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-
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-
1
-
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-
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1
1
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1
-
-
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15270
Debussche
Biosynthesis of the corrin mac ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
175
7430-7440
1993
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
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2
1
-
-
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-
-
-
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-
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-
-
-
-
-
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1
1
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1
-
-
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2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3211
Crouzet
Genetic and sequence analysis ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
172
5980-5990
1990
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-
-
-
-
-
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1
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1
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2
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1
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2
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