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Literature summary for 2.1.1.13 extracted from

  • Koutmos, M.; Datta, S.; Pattridge, K.A.; Smith, J.L.; Matthews, R.G.
    Insights into the reactivation of cobalamin-dependent methionine synthase (2009), Proc. Natl. Acad. Sci. USA, 106, 18527-18532.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hms174(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method using 0.2 M potassium nitrate, 18% (w/v) PEG3350, or microbatch vapor diffusion method using 0.2 M potassium nitrate, 20% (w/v) PEG3350, 50 mM HEPES pH 7.5 Escherichia coli

Protein Variants

Protein Variants Comment Organism
Y1139F the mutation lowers the cobalamine reduction potential from -490 mV to -540 mV Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P13009
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography using a Hi-Trap and a MonoQ column Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
Escherichia coli tetrahydropteroylmonoglutamate + L-methionine
-
?

Synonyms

Synonyms Comment Organism
cobalamin-dependent methionine synthase
-
Escherichia coli
MetH
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
vitamin B12
-
Escherichia coli